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Series GSE277126

Query DataSets for GSE277126

Status

Public on Sep 25, 2024

Title

Anti-necroptotic effects of human Wharton’s jelly-derived mesenchymal stem cells in skeletal muscle cell death model via secretion of GRO-α

Platform organism

Homo sapiens

Sample organisms

Homo sapiens; Mus musculus

Experiment type

Protein profiling by protein array

Summary

Human mesenchymal stem cells (hMSCs) have therapeutic applications and potential for use in regenerative medicine. However, the use of hMSCs in research and clinical medicine is limited by a lack of information pertaining to their donor-specific functional attributes. In this study, we compared the characteristics of same-donor derived placenta (PL) and Wharton’s jelly (WJ)-derived hMSCs, we also compared their mechanism of action in a skeletal muscle disease in vitro model. The effects of GRO-α were also investigated in a Duchenne muscular dystrophy (DMD) mouse model. The same-donor-derived hWJ- and hPL-MSCs exhibited typical hMSC characteristics. GRO-α was differentially expressed in hWJ- and hPL-MSCs. However, when the secretion of GRO-α was considered, hWJ-MSCs displayed a higher ability to inhibit necroptosis in skeletal muscle cells than hPL-MSCs. This demonstrates the anti-necroptotic therapeutic effect of GRO-α in the skeletal muscle cell death model. Furthermore, considering their potential to inhibit necroptosis in skeletal muscle cells, hWJ-MSCs and the derived GRO-α are novel treatment options for skeletal muscle diseases such as DMD.

Overall design

To identify the secreted anti-necroptosis soluble factor from hWJ-MSCs, the conditioned media from each sample group, comprising hWJ- and hPL-MSCs from the same donor co-cultured with C2C12 cells, were analyzed using antibody arrays. We collected the conditioned media from the following groups to perform the array: C2C12 alone, hWJ-MSCs alone (A, B, C), hPL-MSCs alone (A, B, C), and C2C12 cells co-cultured with hWJ-MSCs (A, B, C), or hPL-MSCs (A, B, C). To identify the secreted anti-necroptosis soluble factor from hWJ-MSCs, the conditioned media from each sample group, comprising hWJ- and hPL-MSCs from the same donor co-cultured with C2C12 cells, were analyzed using antibody arrays.

Contributor(s)

Park S, Kwon S, Kim S, Jeong J, Kim M, Choi S, Oh S, Ryu G, Jeon H, Chang J

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Submission date

Sep 13, 2024

Last update date

Sep 26, 2024

Contact name

Sun Jeong Kim

E-mail(s)

[email protected]