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| Status |
Public on Nov 12, 2024 |
| Title |
Integrated multi-modal analysis reveals tumor and immune characteristics that distinguish Epstein-Barr virus-positive and -negative B cell post-transplant lymphoproliferative disorders [Affymetrix] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The highly prevalent oncogenic Epstein-Barr virus (EBV) can drive tumorigenesis with disrupted host immunity, causing malignancies including post-transplant lymphoproliferative disorders (PTLD). PTLD can also arise in the absence of EBV but the biological differences underlying EBV(+) and EBV(-) B cell PTLD and the associated host-EBV-tumor interactions remain poorly understood. Here, we reveal the core differences between EBV(+) and EBV(-) PTLD, characterized by increased expression of genes related to immune processes or DNA interactions respectively, and the augmented ability of EBV(+) PTLD B cells to modulate the tumor microenvironment through elaboration of monocyte-attracting cytokines/chemokines. We create a reference resource of proteins distinguishing EBV(+) B lymphoma cells from EBV(-) B lymphoma including the immunomodulatory molecules CD300a and CD24, respectively. Moreover, we show that CD300a is essential for maximal survival of EBV(+) PTLD B lymphoma cells. Our comprehensive multi-modal analyses uncover the biological underpinnings of PTLD and offer opportunities for precision therapies.
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| Overall design |
Microarrays were used to elucidate the B cell intrinsic differences between B cells transformed with EBV and B cells transformed in the absence of EBV. Specifically, we profiled the transcriptome of five EBV(+) B cell lymphoma lines derived from patients with EBV(+) PTLD with morphology corresponding to diffuse large B cell lymphoma (DLBCL) (AB5, JB7, JC62, MF4, VB5), and two EBV(-) DLBCL cell lines (Pfeiffer, Toledo).
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| Contributor(s) |
Toh J |
| Citation missing |
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| |
| Submission date |
Oct 16, 2024 |
| Last update date |
Nov 13, 2024 |
| Contact name |
Jiaying Toh |
| Organization name |
Stanford University
|
| Street address |
1201 Welch Road
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| City |
Stanford |
| State/province |
California |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
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| Samples (7)
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| This SubSeries is part of SuperSeries: |
| GSE279991 |
Integrated multi-modal analysis reveals tumor and immune characteristics that distinguish Epstein-Barr virus-positive and -negative B cell post-transplant lymphoproliferative disorders |
|
| Relations |
| BioProject |
PRJNA1173572 |
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