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| Status |
Public on Nov 05, 2024 |
| Title |
The role of the LysR-type transcription factor PacR in regulating nitrogen metabolism in Anabaena sp. PCC7120 |
| Organism |
Nostoc sp. PCC 7120 = FACHB-418 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Deletion of the global transcription factor PacR in Anabaena triggers heterocyst formation even in NO3- containing medium, likely due to impaired NO3- uptake and disrupted NH4+ assimilation in the GOGAT cycle. This phenotype may be exacerbated by reduced PSI-yield and reduced expression of ferredoxin, which may lead to less reducing equivalents for nitrogen uptake and fixation. These results highlight PacR’s role as a global regulator of carbon metabolism and photosynthesis while also establishing its involvement in regulating nitrogen metabolism.
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| Overall design |
To investigate the effect of PacR-deletion in Anabaena sp. PCC7120, we used a ΔpacR strain and a control strain which expressed PacR. We took samples directly before changing the nitrogen source from NH4+ to NO3- as well as 1h after and 12h after the shift. RNA was extracted from 3 biological replicates per timepoint and strain. We then performed differential expression anaysis between the strains and between the timepoints for each strain.
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| Web link |
https://doi.org/10.1101/2024.11.01.621236
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| Contributor(s) |
Werner EM, Huokko T, Nikkanen L, Santana-Sánchez A, Koch B, Herrero A, Allhaverdiyeva Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Oct 27, 2024 |
| Last update date |
Nov 06, 2024 |
| Contact name |
Yagut Allahverdiyeva |
| E-mail(s) |
[email protected]
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| Organization name |
Turku University
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| Department |
Life Sciences
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| Lab |
Photomicrobes
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| Street address |
Tykistönkatu 6A
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| City |
Turku |
| ZIP/Postal code |
20520 |
| Country |
Finland |
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| Platforms (1) |
| GPL35023 |
DNBSEQ-G400 (Nostoc sp. PCC 7120 = FACHB-418) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA1178285 |
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