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| Status |
Public on Mar 25, 2014 |
| Title |
Expression data from the mushroom bodies of Drosophila-PKA activity dependent manner |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Temperature preference behavior in Drosophila depends on the level of PKA signaling in the mushroom bodies.
To identify new components downstream to PKA, we carried out a genome-wide screen for genes regulated by PKA signaling in the mushroom bodies.
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| Overall design |
Using the Gal4-UAS system, we increased or decreased PKA activity in the mushroom bodies by expressing dominant-negative (UAS-PKADN) or constitutively active PKA (UAS-PKACA), respectively. Expression of PKA transgenes was targeted to the mushroom bodies using the mushroom body-specific MB247-Gal4 driver. PKA expression was induced for 12-16 hours in three-day-old adults by inactivating the temperature-sensitive Gal80 at the restrictive temperature. We then analyzed gene-expression profiles to identify the genes showing altered expression levels in response to the high or low PKA activity.
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| Contributor(s) |
Kang J, Kim J, Choi K |
| Citation missing |
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| Submission date |
Mar 25, 2011 |
| Last update date |
May 04, 2018 |
| Contact name |
Jongkyun Kang |
| Organization name |
KAIST
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| Street address |
335 Gwahangro, Yuseong-gu
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| City |
Daejeon |
| ZIP/Postal code |
305-701 |
| Country |
South Korea |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA139535 |
