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| Status |
Public on Nov 20, 2024 |
| Title |
Single-cell RNA sequencing of human IL-18R supported CAR T cells targeting oncofetal Tenascin C |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
C.TNC-CAR.Zip18R CAR-T cell of 2 donors were used for 2 in vitro experiments. In the first experiment, we did scRNA-seq for activated CAR-T cells. In the second experiment, the CAR-T cells of each donor were cocultured with tumor cells for 12 hrs, 24 hrs and 48 hrs. The CAR-T cells for each timepoints were extracted for scRNA-seq. Multiplexed 10x Genomics scRNA-seq were performed with a hashtag to label each sample.
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| Overall design |
The experiments were designed to compare the dynamic of cell population size and enriched pathway in different cell populations.
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Raw files for human/patient samples are being made available in EGA (https://www.ebi.ac.uk/ega/) for controlled access to the personally identifiable sequence data.
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| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 15, 2024 |
| Last update date |
Nov 20, 2024 |
| Contact name |
Liqing Tian |
| E-mail(s) |
[email protected]
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| Organization name |
St. Jude Children's Research Hospital
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| Street address |
262 Danny Thomas Pl
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
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| Platforms (1) |
| GPL34284 |
Illumina NovaSeq X Plus (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA1186711 |
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