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| Status |
Public on Apr 30, 2011 |
| Title |
Genome-wide profiling of miRNAs and other small non-coding RNAs in the Verticillium dahliae inoculated cotton roots |
| Organisms |
Gossypium barbadense; Gossypium hirsutum |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) are short (19â25 nucleotides) non-coding RNA molecules that have large-scale regulatory effects on development and on stress responses in plants.The objective of this study is to investigate the transcriptional profile of miRNAs and other small non-coding RNAs in Verticilliumâinoculated cotton roots. Four small RNA libraries were constructed from mocked and infected roots of two cotton cultured species which are with different Verticillium tolerance (âHai-7124â, Gossypium barbadense L., a Verticillium-tolerant cultivar, and âYi-11â, Gossypium hirsutum L. a Verticillium-sensitive cultivar). The length distribution of obtained small RNA pools was significantly different among libraries. A total of 215 conserved miRNA families were identified in the two cotton species, of them 14 are novel. There were >65 families with different expression between two libraries. We also identified two ta-siRNAs and thousands of endogenous siRNA candidates, and hundred of them exhibited altered expression after inoculation of Verticillium. The profiling of these miRNAs and other small non-coding RNAs lay the foundation for further understanding of small RNAs function in the regulation of Verticillium defence responses in cotton roots.
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| Overall design |
Examination of 2 different traetments in 2 cotton types.
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| Contributor(s) |
Yin Z, Shen F |
| Citation missing |
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| |
| Submission date |
Mar 29, 2011 |
| Last update date |
Jun 11, 2013 |
| Contact name |
Zujun Yin |
| E-mail(s) |
[email protected]
|
| Phone |
86-0372-2562279
|
| Organization name |
Chinese Academy of Agricultural Sciences
|
| Department |
Cotton Research Institute
|
| Lab |
State Key Laboratory of Cotton Biology
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| Street address |
38 Huanghe Dadao, Anyang 455000, Henan, China
|
| City |
Anyang |
| State/province |
Henan |
| ZIP/Postal code |
455000 |
| Country |
China |
| |
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| Platforms (2) |
| GPL9362 |
Illumina Genome Analyzer (Gossypium hirsutum) |
| GPL11270 |
Illumina Genome Analyzer (Gossypium barbadense) |
|
| Samples (4)
|
| GSM699077 |
G. barbadense Verticillium inoculated |
|
| Relations |
| BioProject |
PRJNA139607 |
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