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| Status |
Public on Nov 27, 2024 |
| Title |
Thyroid hormone-responsive genes in primary cultures of rat hepatic cells |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
cDNA microarray analysis was applied to primary cultures of rat hepatic cells treated with triiodothyronine (T3) at 10-9 M for 24 hrs to identify the differentially expressed genes. A limited number of genes were listed, and only 3 of them, pyridoxal kinase (Pdxk), phosphoe-nolpyruvate carboxykinase 1 (Pck1), and solute carrier family 17 member 2 (Slc17a2), were con-firmed to be up-regulated by quantitative RT-PCR.
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| Overall design |
Primary cultured rat hepatocytes were treated with or without T3 at 10-9 M for 24 h. Total RNA was extracted with NucleoSpin RNA II. Total RNA from control and T3-treated cells was subjected to cDNA microarray analysis using a GeneChip Array (Rat Genome 230 2.0).
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| Contributor(s) |
Fujimoto N, Kitamura S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Nov 22, 2024 |
| Last update date |
Nov 27, 2024 |
| Contact name |
Nariaki FUJIMOTO |
| E-mail(s) |
[email protected]
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| Phone |
+81822575820
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| Organization name |
Hiroshima University
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| Department |
RIRBM
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| Street address |
1-2-3 Kasumi
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| City |
Hiroshima |
| State/province |
Hiroshima |
| ZIP/Postal code |
7340037 |
| Country |
Japan |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1189567 |
