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| Status |
Public on Nov 27, 2024 |
| Title |
Bulk RNA-seq of pericytes isolated from mouse brain and heart |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goal of the experiment was to characterize the transcriptome of Pericytes from mouse brain and heart. Data were analyzed to examine the ion channels, exchangers and pumps that are enriched in pericytes
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| Overall design |
Pdgfr-β-CreERT2 transgenic mice (Jackson: 029684) were crossed with Ai14 reporter mice (Jackson #007914) that express tdTomato fluorescence following Cre-mediated recombination. Offspring were treated with tamoxifen, which led to robust tdTomato expression in pericytes. Pericytes were isolated by enzymatic digestion from the brains and hearts of 5 mice, subjected to cell sorting and bulk RNA-sequencing.
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| Citation missing |
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| |
| Submission date |
Nov 26, 2024 |
| Last update date |
Nov 27, 2024 |
| Contact name |
William Coetzee |
| E-mail(s) |
[email protected]
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| Organization name |
NYU School of Medicine
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| Department |
Pathology
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| Street address |
550 First Ave
|
| City |
New York |
| State/province |
United States |
| ZIP/Postal code |
10514 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA1191030 |
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