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| Status |
Public on Jan 06, 2012 |
| Title |
Normal human osteoblasts and Hs578t mono and coculture |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
As a model for investigating changes in gene expression in response to epithelial-osteoblast interactions in bone metastases of breast carcinomas, cells that represented malignant epithelial cell compartments (Hs578T) and cells that represented skeletal compartments (Normal Human (NH) Osteoblasts) were examined in an in vitro mixed co-culture setting. These two types of cells were co-cultivated for 48 h in a low-serum medium [0.2% fetal bovine serum (FBS)] to allow reciprocal signal exchange with minimal background from undefined molecular signals that are inherent in fetal bovine serum. We examined the effects of co-cultivation on each cell pairing in two independent biological replicates. The gene expression profiles of the co-cultures were compared to the expression profiles of the corresponding cells that were kept in monoculture using HEEBO microarrays. After mono or co-culturing, total RNA was extracted and amplified using a modified Eberwine procedure. The amplified RNA was labeled with the fluorescent dye Cy5 and pooled with Cy3 labeled reference RNA, and then the pooled RNA was hybridized onto HEEBO microarrays. After hybridization and washing, arrays were scanned on a fluorescent microscope scanner.
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| Overall design |
An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract.
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| Contributor(s) |
Rajski M, Reddy T |
| Citation(s) |
22235336 |
| |
| Submission date |
May 03, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
Michal Rajski |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Zurich
|
| Department |
Institute of Physiology
|
| Lab |
23K86
|
| Street address |
Winterthurerstrasse 190
|
| City |
Zurich |
| ZIP/Postal code |
CH - 8057 |
| Country |
Switzerland |
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| Platforms (1) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE29036 |
Global gene expression analysis of heterotypic interactions between cancer cells and osteoblasts in vitro |
|
| Relations |
| BioProject |
PRJNA142925 |
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