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| Status |
Public on May 01, 2012 |
| Title |
Global transgenerational gene expression dynamics in two nascent allohexaploid wheat lines analogous in genome constitution to common wheat (Triticum aestivum) |
| Platform organism |
Triticum aestivum |
| Sample organisms |
Triticum aestivum; Triticum turgidum subsp. durum; Aegilops tauschii; Triticum carthlicum |
| Experiment type |
Expression profiling by array
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| Summary |
Alteration in gene expression accompanying initial stages of allopolyploidy is a prominent feature in plants, but its spectrum and model are highly idiosyncratic. We used multi-colour GISH to identify individuals from two nascent allohexaploid wheat lines between Triticum turgidum and Aegilops tauschii, which had a transgenerationally stable chromosomal constitution mimicking that of common wheat. We performed genomewide analysis of gene expression for these plants along with their parental species using the Affymetrix GeneChip Wheat Genome-Array. Comparison with parental species coupled with inclusion of empirical mid-parent values (MPVs) revealed two patterns of alteration in gene expression in the allohexaploid lines: parental dominance expression and nonadditive expression. Genes involved in each altered pattern could be classified into three distinct groups, stochastic, heritable and persistent, based on their transgenerational heritability and inter-line conservation. Whereas both altered patterns of gene expression showed a propensity of inheritance, identity of the involved genes is stochastic, consistent with the involvement of diverse Gene Ontology (GO) terms. Nonetheless, those genes showing nonadditive expression exhibited a significant enrichment for vesicle-function. Our results suggest global alteration in gene expression conditioned by nascent allopolyploidy likely play functional roles in stabilization and establishment of the newly formed plants, and consequential to evolution.
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| Overall design |
We used Affymetrix GeneChip Wheat Genome-Array to compare the expression difference of newly synthetic wheat allopolyploid lines and their parental lines.
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| Contributor(s) |
Qi B, Huang W, Zhu B, Zhong X, Guo J, Zhao N, Xu C, Pang J, Han F, Feldman M, Liu B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jun 10, 2011 |
| Last update date |
Dec 04, 2018 |
| Contact name |
bao qi |
| E-mail(s) |
[email protected]
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| Organization name |
chinese academy of science
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| Street address |
5268 Renmin Street
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| City |
changchun |
| ZIP/Postal code |
130024 |
| Country |
China |
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| Platforms (1) |
| GPL3802 |
[wheat] Affymetrix Wheat Genome Array |
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| Samples (18)
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| GSM740062 |
Leaf of AT5 at selfed generation 4, biological rep1 |
| GSM740063 |
Leaf of AT5 at selfed generation 4, biological rep2 |
| GSM740064 |
Leaf of AT5 at selfed generation 5, biological rep1 |
| GSM740065 |
Leaf of AT5 at selfed generation 5, biological rep2 |
| GSM740066 |
Leaf of AT9 at selfed generation 4, biological rep1 |
| GSM740067 |
Leaf of AT9 at selfed generation 4, biological rep2 |
| GSM740068 |
Leaf of AT9 at selfed generation 5, biological rep1 |
| GSM740069 |
Leaf of AT9 at selfed generation 5, biological rep2 |
| GSM740070 |
Leaf of MPV5, biological rep1 |
| GSM740071 |
Leaf of MPV5, biological rep2 |
| GSM740072 |
Leaf of MPV9, biological rep1 |
| GSM740073 |
Leaf of MPV9, biological rep2 |
| GSM740074 |
Leaf of TQ27, biological rep1 |
| GSM740075 |
Leaf of TQ27, biological rep2 |
| GSM740076 |
Leaf of TTH01, biological rep1 |
| GSM740077 |
Leaf of TTH01, biological rep2 |
| GSM740078 |
Leaf of TTR04, biological rep1 |
| GSM740079 |
Leaf of TTR04, biological rep2 |
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| Relations |
| BioProject |
PRJNA140735 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE29882_RAW.tar |
77.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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