|
| Status |
Public on Jun 14, 2012 |
| Title |
tRNA gene identity acts as a determinant of gene positioning |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
|
| Summary |
Here we report that the spatial organization of yeast tRNA genes depends upon both locus position and tRNA identity; supporting the idea that the genomic organization of tRNA loci utilizes tRNA dependent signals within the nucleoprotein-tRNA complexes that form into clusters. We use high-throughput sequencing coupled to Circular Chromosome Conformation Capture to detect interactions with two wild type tRNAs and these same positions replaced with suppressor tRNAs (SUP4-1).
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| Overall design |
Detect DNA-DNA interactions (Circular chromosome conformation capture; 4C) with two wild type tRNAs and these same positions replaced with suppressor tRNAs (SUP4-1)
Supplementary files:
Alignment files generated by Topography v1.19 software.
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| |
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| Contributor(s) |
O'Sullivan JM, Engelke D, Pai D, Rodley CD |
| Citation(s) |
22206006 |
| |
| Submission date |
Jun 20, 2011 |
| Last update date |
Nov 21, 2014 |
| Contact name |
Justin M. O'Sullivan |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Auckland
|
| Department |
Liggins Institute
|
| Street address |
85 Park Road, Grafton
|
| City |
Auckland |
| ZIP/Postal code |
1023 |
| Country |
New Zealand |
| |
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| Platforms (1) |
| GPL9134 |
Illumina Genome Analyzer (Saccharomyces cerevisiae) |
|
| Samples (8)
|
| GSM745463 |
4C PCR of tL(CAA)G3 tRNA containing MspI fragment |
| GSM745464 |
4C PCR of tL(UAA)B2 tRNA containing MspI fragment |
| GSM745465 |
Fragment 1 4C PCR of tL(CAA)G3 tRNA replaced with SUP4-1 containing MspI fragment |
| GSM745466 |
Fragment 2 4C PCR of tL(CAA)G3 tRNA replaced with SUP4-1 containing MspI fragment |
| GSM745467 |
Fragment 1 4C PCR of tL(UAA)B2 tRNA replaced with SUP4-1 containing MspI fragment |
| GSM745468 |
Fragment 2 4C PCR of tL(UAA)B2 tRNA replaced with SUP4-1 containing MspI fragment |
| GSM786062 |
Saccharomyces_cerevisiae_Glucose_GCC_Rep1 |
| GSM786063 |
Saccharomyces_cerevisiae_Glucose_GCC_Rep2 |
|
| Relations |
| BioProject |
PRJNA143893 |
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