|
| Status |
Public on Jul 28, 2011 |
| Title |
Microarray Analysis of West Nile Virus infected Human Retinal Pigment Epithelium |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Low-level infection is believed to play a role in the degradation of the outer blood retinal barrier, which is composed of retinal pigment epithelial (RPE) cells.
By investigating immunopathogenic West nile virus (WNV) infected RPE via microarray, we sought to find key genes involved in a low-level viral infection, which are vital for normal immune responses.
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| |
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| Overall design |
We infected primary human RPE cells and an human RPE cell line with WNV and extracted RNA to hybridize onto Affymetrix arrays. We were interested in the global expression of differentially regulated genes in WNV infected RPE cells, at 24h post infection. We were also interested in seeing the differences in response between infected primaries and the infected ARPE19 cell line.
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| |
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| Contributor(s) |
Munoz-Erazo L, Natoli R, Provis JM, Madiagn MC, King NJ |
| Citation(s) |
22509103 |
| |
| Submission date |
Jul 15, 2011 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Luis Munoz-Erazo |
| E-mail(s) |
[email protected]
|
| Phone |
+61293516157
|
| Organization name |
University of Sydney
|
| Department |
Department of Pathology
|
| Lab |
Viral Immunopathology Unit
|
| Street address |
Room 260, Blackburn building D06
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2006 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (10)
|
| GSM762060 |
uninfected primary RPE, biological replicate 1 |
| GSM762061 |
infected primary RPE, biological replicate 1 |
| GSM762062 |
uninfected primary RPE, biological replicate 2 |
| GSM762063 |
infected primary RPE, biological replicate 2 |
| GSM762064 |
uninfected primary RPE, biological replicate 3 |
| GSM762065 |
infected primary RPE, biological replicate 3 |
| GSM762066 |
uninfected primary RPE, biological replicate 4 |
| GSM762067 |
infected primary RPE, biological replicate 4 |
| GSM762068 |
uninfected ARPE19 cell line |
| GSM762069 |
infected ARPE19 cell line |
|
| Relations |
| BioProject |
PRJNA144383 |
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