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| Status |
Public on Mar 27, 2012 |
| Title |
Rice gene global expression analysis upon inoculation with different Magnaporthe isolates |
| Organism |
Oryza sativa |
| Experiment type |
Expression profiling by array
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| Summary |
Magnaporthe oryzae is the causative agent of the rice blast, the most relevant rice disease worldwide. To date expression analysis on rice infected with Magnaporthe oryzae have been carried out only with the strains FR13 (leaf) and Guy 11 (root). However different strains of Magnaporthe are present in the environment leading to different rice responses at molecular level. To gain more insight on the unknown molecular mechanisms activated by different Magnaporthe strains during rice defense, a global expression analysis was performed by using the GeneChip® Rice Genome Array.
To identify rice genes differentially regulated upon infection by Magnaporthe isolates, inoculation with different strains were performed and samples were collected 24 hours post infection.
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| Overall design |
RNA were obtained from leaf samples after inoculation of rice 2 week-old plantlets with the following strains: rice isolates Magnaporthe oryzae FR13 and CL367, non-adapted strain BR32, isolated from wheat, and Magnaporthe grisea BR29 isolated from crabgrass. Treated and control (mock) rice leaves (cv. Nipponbare) were collected 24 hours post inoculation. Three biological replicates for each interaction type and the corresponding mock were extracted and analysed independently with the GeneChip® Rice Genome Array.
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| Contributor(s) |
Abbruscato P, Faivre-Rampant O, Del Corvo M |
| Citation(s) |
22443363 |
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| Submission date |
Jul 26, 2011 |
| Last update date |
Dec 27, 2017 |
| Contact name |
PAMELA ABBRUSCATO |
| E-mail(s) |
[email protected]
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| Phone |
+3903714662623
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| Fax |
+3903714662217
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| URL |
http://www.tecnoparco.or/ricegenomics
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| Organization name |
PARCO TECNOLOGICO PADANO
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| Department |
RICE GENOMICS UNIT
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| Street address |
via Einstein
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| City |
LODI |
| State/province |
LO |
| ZIP/Postal code |
26900 |
| Country |
Italy |
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| Platforms (1) |
| GPL2025 |
[Rice] Affymetrix Rice Genome Array |
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| Samples (15)
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| GSM767204 |
BR29 at 24 hpi, biological replicate 1 |
| GSM767205 |
BR29 at 24 hpi, biological replicate 2 |
| GSM767206 |
BR29 at 24 hpi, biological replicate 3 |
| GSM767207 |
BR32 at 24 hpi, biological replicate 1 |
| GSM767208 |
BR32 at 24 hpi, biological replicate 2 |
| GSM767209 |
BR32 at 24 hpi, biological replicate 3 |
| GSM767210 |
CL367 at 24 hpi, biological replicate 1 |
| GSM767211 |
CL367 at 24 hpi, biological replicate 2 |
| GSM767212 |
CL367 at 24 hpi, biological replicate 3 |
| GSM767213 |
FR13 at 24 hpi, biological replicate 1 |
| GSM767214 |
FR13 at 24 hpi, biological replicate 2 |
| GSM767215 |
FR13 at 24 hpi, biological replicate 3 |
| GSM767216 |
mock at T24 hpi, biological replicate 1 |
| GSM767217 |
mock at T24 hpi, biological replicate 2 |
| GSM767218 |
mock at T24 hpi, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA146297 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE30941_RAW.tar |
69.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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