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Status |
Public on Oct 17, 2011 |
Title |
Integrated transcriptomic and proteomic profiling of white spruce stems during the transition from active growth to dormancy. |
Organism |
Picea glauca |
Experiment type |
Expression profiling by array
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Summary |
In the autumn, stems of woody perennials such as forest trees undergo a transition from active growth to dormancy. We used microarray transcriptomic profiling in combination with a proteomics analysis to elucidate processes that occur during this growth-to-dormancy transition in a conifer, white spruce (Picea glauca [Moench] Voss). Several differentially expressed genes were likely associated with the developmental transition that occurs during growth cessation in the cambial zone and the concomitant completion of cell maturation in vascular tissues. Genes encoding for cell wall and membrane biosynthetic enzymes showed transcript abundance patterns consistent with completion of cell maturation, and also of cell wall and membrane modifications potentially enabling cells to withstand the harsh conditions of winter. Several differentially expressed genes were identified that encoded putative regulators of cambial activity, cell development, and of the photoperiodic pathway. Reconfiguration of carbon allocation figured centrally in the tree’s overwintering preparations. For example, genes associated with carbon-based defenses such as terpenoids were downregulated, while many genes associated with proteinbased defenses and other stress mitigation mechanisms were upregulated. Several of these correspond to proteins that were accumulated during the growth-to-dormancy transition, emphasizing the importance of stress protection in the tree’s adaptive response to overwintering.
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Overall design |
Two year old white spruce (Picea glauca [Moench] Voss) seedlings were used for all experiments, which were conducted as described by El Kayal et al. (2011). Using a complete randomized block design, seedlings were grown in growth chambers under long days (LD; 16h day / 8h night, 20°C, 50 to 60% RH) for 8-10 weeks. Shortly before seedlings were to begin bud formation, the photoperiod was changed to short days (SD; 8h day / 16h night, 20°C, 50 to 60% RH) to induce rapid and synchronous bud formation. This was designated Day 0. Lignified whole stems representing the current year’s growth (microarrays, microscopy) or previous year’s growth (protein analyses) were sampled at 0, 3, 7, 14, 28 and 70 d (10 wk) SD, and immediately frozen in liquid nitrogen. Remaining plants were maintained in SD for an additional 8-15 wk, and then transferred to low temperatures (LT, 2 - 4°C) for 3 to 4 weeks with continuing SD prior to harvest. These are referred to as LT samples.
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Contributor(s) |
Galindo González LM, El Kayal W, Ju CJ, Allen CC, King-Jones S, Cooke JE |
Citation(s) |
21988609 |
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Submission date |
Aug 01, 2011 |
Last update date |
Mar 23, 2012 |
Contact name |
Chelsea Ju |
E-mail(s) |
[email protected]
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Organization name |
University of Alberta
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Department |
Biological Sciences
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Lab |
Dr. Janice Cooke
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Street address |
CW 405, Biological Sciences Centre
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City |
Edmonton |
State/province |
Alberta |
ZIP/Postal code |
T6G 2E9 |
Country |
Canada |
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Platforms (1) |
GPL10754 |
Arborea spruce 11K cDNA microarray |
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Samples (4)
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Relations |
BioProject |
PRJNA144705 |
Supplementary file |
Size |
Download |
File type/resource |
GSE31090_RAW.tar |
48.8 Mb |
(http)(custom) |
TAR (of GPR) |
Processed data included within Sample table |
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