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| Status |
Public on Aug 11, 2014 |
| Title |
Nanog-induced intestinal and colonic hyperplasia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Though expression of the homeobox transcription factor Nanog is generally restricted to pluripotent cells and early germ cells, recent reports have found that Nanog is re-expressed in somatic and germ cell tumors associated with poor differentiation and aggressive disease progression.
To elucidate its oncogenic properties, a modified Tet-On system was utilised to generate Nanog-inducible mice.
By dexamethasone and doxycycline injection, similar Nanog expression levels as in wild-type embryonic stem cells were obtained in all major organs except for brain.
Ectopic Nanog expression resulted in intestinal and colonic epithelium hyperplasia-intestinal villi had doubled in length and hyperplastic epithelium outgrowths were seen after 7 days.
The tumor suppressor gene Cdx2 was downregulated at onset of Nanog expression, suggesting that crosstalk between Nanog and Cdx2 is conserved from early embryo development to adulthood.
Although the mice died before they could form tumors, Cdx2 repression and intestinal hyperplasia links Nanog to tumor initiation.
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| |
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| Overall design |
8 samples were analyzed.
Intestine-: Mouse intestine cells without Nanog overexpression, 2 biological rep
Intestine+: Mouse intestine cells with Nanog overexpression, 2 biological rep
Colon-: Mouse colon cells without Nanog overexpression, 2 biological rep
Colon+: Mouse colon cells with Nanog overexpression, 2 biological rep
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| |
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| Contributor(s) |
Fishedick G, Wu G, Greber B, Araúzo-Bravo MJ, Köhler G, Tapia N, Iacone R, Anastassiadis K, Zaehres H, Schöler HR |
| Citation(s) |
25173648 |
| |
| Submission date |
Aug 16, 2011 |
| Last update date |
Jan 15, 2022 |
| Contact name |
Marcos J. Araúzo-Bravo |
| E-mail(s) |
[email protected]
|
| Phone |
+34 943 00 6108
|
| Organization name |
Max Planck Institute for Molecular Biomedicine
|
| Department |
Cell and Developmental Biology
|
| Lab |
Computational Biology and Bionformatics
|
| Street address |
Rogentstrasse
|
| City |
Muenster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (8)
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| Relations |
| BioProject |
PRJNA145745 |
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