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| Status |
Public on Jun 05, 2024 |
| Title |
Positional Identity of Branching-associated HOXB2 and B3 Gene Expression is Maintained by Adult Human Airway Epithelial Progenitor Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In the context that HOX mutant mice have profound deficits in airway branching morphogenesis, we asked whether the positional identity of HOX genes is maintained in the epithelium of the adult human tracheobronchial tree and if so, whether these positional differences are embedded in the stem/progenitor population derived from nonbranching vs branching airways. We found that 10 of 39 HOX genes are expressed in varying degrees in the nonbranching (trachea) or branching (large and small airways) human airway epithelium. Strikingly, HOXB2 and B3 were highly expressed in the trachea epithelium, but barely detectable in the branching airway epithelium. This difference in HOXB2 and B3 gene expression was embedded in the airway basal cell stem/progenitor population isolated from the trachea vs branching airways, and maintained during differentiation in vitro. Finally, HOXB2 and B3 expressions in the trachea vs branching airways correlated with the expression of a variety of other transcription and growth factors related to branching morphogenesis. The finding that the adult human airway epithelium expresses some HOX genes in a positional manner, with differences embedded in the transcriptomes of airway epithelial stem/progenitor cells, has implications for the use of stem/progenitor cells for applications relevant to lung regeneration.
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| Overall design |
Homeobox (HOX) genes are transcription regulators that mediate embryonic morphogenesis and determine positional identity. Microarrays showed that 9 of 39 HOX genes are expressed in varying degrees in the nonbranching (trachea) or branching (large and small airways) human airway epithelium. Most strikingly, HOXB2 and B3 were highly expressed in the trachea epithelium, but barely detectable in the epithelium from branching airways. This difference was not affected by cigarette smoking, but was embedded in the airway stem/progenitor population and maintained during differentiation. The finding has significant implications for the applications of stem/progenitor cells relevant to lung regeneration and repair.
Examination of nonbranching (trachea) or branching (large and small airways) human airway epithelium+/- cigarette smoke.
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| Contributor(s) |
Wang R, Wang G, Ahmed J, Strulovici-Barel Y, Salit J, Crystal RG |
| Citation missing |
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| Submission date |
Aug 17, 2011 |
| Last update date |
Jun 05, 2024 |
| Contact name |
Yael Strulovici-Barel |
| E-mail(s) |
[email protected]
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| Organization name |
Weill Cornell Medical College
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| Department |
Department of Genetic Medicine
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| Lab |
Crystal
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| Street address |
1300 York Avenue
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (60)
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| Relations |
| BioProject |
PRJNA145821 |
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