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| Status |
Public on Aug 24, 2005 |
| Title |
Two yeast forkhead genes regulate the cell cycle and pseudohyphal growth |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
There are about 800 genes in Saccharomyces cerevisiae whose transcription is cell-cycle regulated. Some of these form clusters of co-regulated genes. The 'CLB2' cluster contains 33 genes whose transcription peaks early in mitosis, including CLB1, CLB2, SWI5, ACE2, CDC5, CDC20 and other genes important for mitosis. Here we find that the genes in this cluster lose their cell cycle regulation in a mutant that lacks two forkhead transcription factors, Fkh1 and Fkh2. Fkh2 protein is associated with the promoters of CLB2, SWI5 and other genes of the cluster. These results indicate that Fkh proteins are transcription factors for the CLB2 cluster. The fkh1 fkh2 mutant also displays aberrant regulation of the 'SIC1' cluster, whose member genes are expressed in the M-G1 interval and are involved in mitotic exit. This aberrant regulation may be due to aberrant expression of the transcription factors Swi5 and Ace2, which are members of the CLB2 cluster and controllers of the SIC1 cluster. Thus, a cascade of transcription factors operates late in the cell cycle. Finally, the fkh1 fkh2 mutant displays a constitutive pseudohyphal morphology, indicating that Fkh1 and Fkh2 may help control the switch to this mode of growth.
Groups of assays that are related as part of a time series.
Keywords: time_series_design
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| Overall design |
Computed
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| Web link |
http://genome-www.stanford.edu/fkh
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| Contributor(s) |
Spellman P |
| Citation(s) |
10894548 |
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| Submission date |
Aug 23, 2005 |
| Last update date |
Jan 18, 2013 |
| Organization |
Stanford Microarray Database (SMD) |
| E-mail(s) |
[email protected]
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| Phone |
650-498-6012
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| URL |
http://genome-www5.stanford.edu/
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| Department |
Stanford University, School of Medicine
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| Street address |
300 Pasteur Drive
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (2) |
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| Samples (26)
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| GSM71617 |
cell cycle-alpha factor, fkh1, fkh2 090min |
| GSM71618 |
cell cycle-alpha factor, fkh1, fkh2 165min |
| GSM71619 |
cell cycle-alpha factor, fkh1, fkh2 150min |
| GSM71620 |
cell cycle-alpha factor, fkh1, fkh2 075min |
| GSM71621 |
cell cycle-alpha factor, fkh1, fkh2 060min |
| GSM71622 |
cell cycle-alpha factor, fkh1, fkh2 120min |
| GSM71623 |
cell cycle-alpha factor, fkh1, fkh2 045min |
| GSM71624 |
fkh1,2 alpha factor 045 min |
| GSM71625 |
cell cycle-alpha factor, fkh1, fkh2 105min |
| GSM71626 |
cell cycle-alpha factor, fkh1, fkh2 030min |
| GSM71627 |
cell cycle-alpha factor, fkh1, fkh2 015min |
| GSM71628 |
cell cycle-alpha factor, fkh1, fkh2 180min |
| GSM71629 |
cell cycle-alpha factor, fkh1, fkh2 000min |
| GSM71630 |
fkh1,2 alpha factor 210 min |
| GSM71631 |
fkh1,2 alpha factor 000 min |
| GSM71632 |
fkh1,2 alpha factor 015 min |
| GSM71633 |
fkh1,2 alpha factor 030min |
| GSM71634 |
fkh1,2 alpha factor 165 min |
| GSM71635 |
fkh1,2 alpha factor 060 min |
| GSM71636 |
fkh1,2 alpha factor 075 min |
| GSM71637 |
fkh1,2 alpha factor 090 min |
| GSM71638 |
fkh1,2 alpha factor 105 min |
| GSM71639 |
fkh1,2 alpha factor 120 min |
| GSM71640 |
fkh1,2 alpha factor 135 min |
| GSM71641 |
cell cycle-alpha factor, fkh1, fkh2 135min |
| GSM71642 |
fkh1,2 alpha factor 150 min |
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| Relations |
| BioProject |
PRJNA92731 |
| Supplementary data files not provided |
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