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| Status |
Public on Sep 08, 2011 |
| Title |
Genome-wide activity-dependent MeCP2 phosphorylation regulates nervous system development and function [cultured cortical neurons] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To examine potential differences in activity-dependent gene expression, we analyzed mRNA expression in cultured neurons isolated from Wild-type vs MeCP2 S421A mice at 0 (unstimulated), 1 or 6 hours after membrane depolarization by exposure to high extracellular KCl (55mM)
All mice were male littermates from one of three litters.
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| Overall design |
We isolated RNA from dissociated cortical cultures (E16+7DIV) isolated from Wild-Type or MeCP2 S421A knock-in mice littermates. Cells were either left unstimulated or depolarized for 1 or 6 hours by addition of 55mM KCl to the media. mRNA expression was analyzed using the Affymetrix GeneChip Mouse Expression Set 430 2.0 microarray platform.
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| Contributor(s) |
Cohen S, Hemberg M, Hutchinson AN, Sadacca LA, Ebert DH, Harmin DA, Greenberg RS, Zhou Z, Wetsel WC, West AE, Gabel HW, Greenberg ME |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 02, 2011 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Harrison Wren Gabel |
| E-mail(s) |
[email protected]
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| Phone |
617-512-0289
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| Organization name |
Harvard Medical School
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| Department |
Neurobiology
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| Lab |
Michael Greenberg
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| Street address |
7 Linden St Apt 3
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| City |
Brookline |
| State/province |
MA |
| ZIP/Postal code |
02445 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE31851 |
Genome-wide activity-dependent MeCP2 phosphorylation regulates nervous system development and function |
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| Relations |
| BioProject |
PRJNA155259 |
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