 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Oct 07, 2011 |
| Title |
Methylation specifies distinct estrogen-induced binding site repertoires of CBP to chromatin (mRNA) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Multiple signaling pathways ultimately modulate the epigenetic information embedded in the chromatin of gene promoters by recruiting epigenetic enzymes. We found that, in estrogen-regulated gene programming, the acetyltransferase CREB-binding protein (CBP) is specifically and exclusively methylated by the coactivator-associated arginine methyltransferase (CARM1) in vivo. CARM1-dependent CBP methylation and p160 coactivators were required for estrogen-induced recruitment to chromatin targets. Notably, methylation increased the histone acetyltransferase (HAT) activity of CBP and stimulated its autoacetylation. Comparative genome-wide chromatin immunoprecipitation sequencing (ChIP-seq) studies revealed a variety of patterns by which p160, CBP, and methyl-CBP (meCBP) are recruited (or not) by estrogen to chromatin targets. Moreover, significant target gene-specific variation in the recruitment of (1) the p160 RAC3 protein, (2) the fraction of a given meCBP species within the total CBP, and (3) the relative recruitment of different meCBP species suggests the existence of a target gene-specific “fingerprint” for coregulator recruitment. Crossing ChIP-seq and transcriptomics profiles revealed the existence of meCBP “hubs” within the network of estrogen-regulated genes. Together, our data provide evidence for an unprecedented mechanism by which CARM1-dependent CBP methylation results in gene-selective association of estrogen-recruited meCBP species with different HAT activities and specifies distinct target gene hubs, thus diversifying estrogen receptor programming.
|
| |
|
| Overall design |
Examination of estrogen-induced transcription in H3396 cells
|
| |
|
| Contributor(s) |
Ceschin DG, Walia M, Wenk SS, Duboé C, Gaudon C, Xiao Y, Fauquier L, Sankar M, Vandel L, Gronemeyer H |
| Citation(s) |
21632823 |
| |
| Submission date |
Sep 23, 2011 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Marco Antonio Mendoza-Parra |
| E-mail(s) |
[email protected]
|
| Organization name |
IGBMC
|
| Street address |
1, rue Laurent Fries
|
| City |
Illkirch; Strasbourg |
| ZIP/Postal code |
67400 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (5)
|
|
| This SubSeries is part of SuperSeries: |
| GSE32350 |
Methylation specifies distinct estrogen-induced binding site repertoires of CBP to chromatin |
|
| Relations |
| BioProject |
PRJNA154903 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE32348_RAW.tar |
21.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...