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| Status |
Public on Mar 05, 2012 |
| Title |
Rap1 Competition-ChIP Galactose Induction Time Course (High Resolution) |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
A strain harboring two copies of RAP1 is used for a competition-ChIP experiment. One copy of RAP1 is expressed from the endogenous RAP1 promoter and a c-terminal 3X FLAG epitiope tag and the other is expressed from a weakened Galactose inducible promoter and a c-terminal 9X MYC tag. Following induction by 2% galactose Rap1-Myc and Rap1-Flag levels are determined genome wide using ChIP-chip.
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| Overall design |
Time Course ChIP-ChIP experiment, Rap1-Flag IP and Rap1-Myc IP. 10 Time Points (0,10,20,30,40,50,60,90,120,150 Minutes) 2 Biological Replicates. Total Rap1 Occupancy at times 0 and 60 minutes in the time course; 2 Biological Replicates. mRNA expression levels at times 0 and 60 minutes in the time course; 2 biological Replicates.
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| Contributor(s) |
Lickwar CR, Lieb JD |
| Citation(s) |
22498630 |
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| Submission date |
Sep 23, 2011 |
| Last update date |
Apr 17, 2012 |
| Contact name |
Jason D Lieb |
| E-mail(s) |
[email protected]
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| Phone |
919-843-3228
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| Organization name |
UNC-Chapel Hill
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| Department |
Biology
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| Lab |
Jason Lieb
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| Street address |
408 Fordham Hal, CB#3280
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| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599-3280 |
| Country |
USA |
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| Platforms (1) |
| GPL14612 |
Nimblegen HD4 Yeast 12X ~270K array [110413_Scer_LI_ChIP] |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA147233 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE32351_Platform_columns_gff_header.txt.gz |
171 b |
(ftp)(http) |
TXT |
| GSE32351_RAW.tar |
296.1 Mb |
(http)(custom) |
TAR (of GFF, PAIR) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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