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| Status |
Public on Nov 11, 2011 |
| Title |
Expression data from mouse B16-F10 cells exposed to hypoxic conditions in vitro. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Analysis of gene expression profile of B16-F10 murine melanoma cells exposed to hypoxic conditions (1% oxygen) or hypoxia mimicry (cobalt chloride) for 24 hours. Gene expression profiles were analyzed using MG-U74Av2 oligonucleotide microarrays. Data analysis revealed 2541 probesets (FDR<5%) for 1% oxygen experiment and 364 probesets (FDR<5%) for cobalt chloride, that showed differences in expression levels. Analysis of hypoxia-regulated genes (1% O2) by stringent Family-Wise Error Rate estimation indicated 454 significantly changed transcripts (p<0.05). The most upregulated genes were Lgals3, Selenbp1, Nppb (more than ten-fold increase). Both hypoxia and hypoxia-mimicry induced HIF-1 regulated genes. However, unsupervised analysis (Singular Value Decomposition) revealed distinct differences between gene expression induced by these two experimental conditions.
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| Overall design |
We investigated transcriptional activity of B16-F10 murine melanoma cells cultured for 24h under hypoxic (nominal 1% oxygen; 9 experimental samples and 6 controls) and hypoxia-mimicking conditions (cobalt chloride, 100 μM or 200 μM, 2 samples each and 2 controls).
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| Contributor(s) |
Olbryt M, Jarząb M, Jazowiecka-Rakus J, Sochanik A |
| Citation(s) |
17193925 |
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| Submission date |
Nov 10, 2011 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Michal Swierniak |
| Organization name |
University of Warsaw
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| Department |
Centre of New Technologies
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| Street address |
Banacha 2C
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| City |
Warsaw |
| ZIP/Postal code |
02-097 |
| Country |
Poland |
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| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
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| Samples (21)
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| GSM830940 |
B16-F10 cells cultured in control conditions (21% oxygen), biological replica 1 |
| GSM830941 |
B16-F10 cells cultured in control conditions (21% oxygen), biological replica 2 |
| GSM830942 |
B16-F10 cells exposed to experimental hypoxia (1% oxygen) in Billups_Rothenberg chamber, biological replica 1 |
| GSM830943 |
B16-F10 cells exposed to experimental hypoxia (1% oxygen) in Billups_Rothenberg chamber, biological replica 2 |
| GSM830944 |
B16-F10 cells exposed to experimental hypoxia (1% oxygen) in Billups_Rothenberg chamber, biological replica 3 |
| GSM830945 |
B16-F10 cells cultured in control conditions (21% oxygen), biological replica 1, experiment 1 |
| GSM830946 |
B16-F10 cells cultured in control conditions (21% oxygen), biological replica 2, experiment 1 |
| GSM830947 |
B16-F10 cells exposed to experimental hypoxia (1% oxygen) in incubator, biological replica 1, experiment 1 |
| GSM830948 |
B16-F10 cells exposed to experimental hypoxia (1% oxygen) in incubator, biological replica 2, experiment 1 |
| GSM830949 |
B16-F10 cells exposed to experimental hypoxia (1% oxygen) in incubator, biological replica 3, experiment 1 |
| GSM830950 |
B16-F10 cells cultured in control conditions (21% oxygen), biological replica 1, experiment 2 |
| GSM830951 |
B16-F10 cells cultured in control conditions (21% oxygen), biological replica 2, experiment 2 |
| GSM830952 |
B16-F10 cells exposed to experimental hypoxia (1% oxygen) in incubator, biological replica 1, experiment 2 |
| GSM830953 |
B16-F10 cells exposed to experimental hypoxia (1% oxygen) in incubator, biological replica 2, experiment 2 |
| GSM830954 |
B16-F10 cells exposed to experimental hypoxia (1% oxygen) in incubator, biological replica 3, experiment 2 |
| GSM830955 |
B16-F10 cells cultured in control conditions (21% oxygen), biological replica 1, experiment 3 |
| GSM830956 |
B16-F10 cells cultured in control conditions (21% oxygen), biological replica 2, experiment 3 |
| GSM830957 |
B16-F10 cells exposed to 100 μM CoCl2, biological replica 1, experiment 3 |
| GSM830958 |
B16-F10 cells exposed to 100 μM CoCl2, biological replica 2, experiment 3 |
| GSM830959 |
B16-F10 cells exposed to 200 μM CoCl2, biological replica 1, experiment 3 |
| GSM830960 |
B16-F10 cells exposed to 200 μM CoCl2, biological replica 2, experiment 3 |
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| Relations |
| BioProject |
PRJNA148531 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE33607_RAW.tar |
54.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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