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| Status |
Public on May 18, 2012 |
| Title |
microRNA expression analysis of circulating monocytes in obese patients |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Human betaherpesvirus 5; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Merkel cell polyomavirus; Mus musculus cytomegalovirus 2; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Low-grade chronic inflammation plays an important role in the development of obesity and obesity-associated disorders such as insulin resistance, type 2 diabetes, the metabolic syndrome and atherosclerosis. One possible link between obesity and inflammation is the enhanced activation of circulating monocytes making them more prone to infiltration into the adipose and vascular tissues of obese persons. microRNAs are a class of small endogenous non-coding RNAs, which function as important regulators of inflammation by modulating gene expression. Therefore, microRNA analysis of circulating monocytes from control and obese patients will potentially provide insights into the pathophysiology of obesity and associated disorders and supply biomarkers for diagnostic purpose. The cohort comprised 6 lean age-matched controls (BMI: 20±0.8 kg/m2, mean±SEM) and 9 obese individuals without clinical symptoms of cardiovascular disease (BMI: 46±1.5 kg/m2, P<0.001 compared with lean controls). CD14+ monocytes were collected, total RNA was extracted and subjected to microRNA expression analysis.
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| Overall design |
Samples consisted of CD14+ monocytes from 6 lean controls and 9 morbidly obese patients.
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| Contributor(s) |
Hulsmans M, Holvoet P |
| Citation(s) |
22535975 |
| |
| Submission date |
Dec 07, 2011 |
| Last update date |
May 18, 2012 |
| Contact name |
Paul Holvoet |
| E-mail(s) |
[email protected]
|
| URL |
http://www.kuleuven.be/amu
|
| Organization name |
K.U.Leuven
|
| Department |
Department of Cardiovascular Diseases
|
| Lab |
Atherosclerosis and Metabolism Unit
|
| Street address |
Herestraat 49, PB 705
|
| City |
Leuven |
| ZIP/Postal code |
3000 |
| Country |
Belgium |
| |
|
| Platforms (1) |
| GPL11432 |
miRCURY LNA microRNA Array, 5th generation - hsa, mmu & rno |
|
| Samples (15)
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| Relations |
| BioProject |
PRJNA149775 |
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