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| Status |
Public on Dec 17, 2011 |
| Title |
High-throughput sequencing of AGO-immunoprecipitating miRs in human senescent fibroblast WI-38 |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
In humans, there are four Ago proteins (Ago1–4) and AGO1- and 2 were previously implicated in TGS induced by exogenous siRNAs and microRNAs (miRs) directed against gene promoter transcripts via promotion of changes in histone covalent modifications and DNA methylation. Not-with-standing, many mechanistic details of this process remain poorly defined in human cells, and very little is known about the identity of possible endogenous signals, which may drive this process in human cells. Given the evolutionary conserved role of siRNAs and AGO proteins in TGS and heterochromatin formation, we set out to analyse their possible involvement in senesence-associated repression of E2F target genes. To obtain a detailed picture of AGO-immunoprecipitating miRs (RIP) in senescent cells, we used next-generation sequencing (NGS)(RIP-Seq). We also included histone H3 dimethylated on lysine 9 (H3K9me2) in this analysis to assign potential AGO2-interacting miRs to a repressive chromatin state and unfractionated, cellular RNA from senescent cells for normalisation.
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| Overall design |
Determination of AGO AGO-immunoprecipitating miRs in WI-38 senescent human fibroblast
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| Contributor(s) |
Bischof O |
| Citation(s) |
22366686 |
| |
| Submission date |
Dec 16, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Moussa BENHAMED |
| E-mail(s) |
[email protected]
|
| Organization name |
Institut Pasteur
|
| Street address |
Institut pasteur
|
| City |
paris |
| ZIP/Postal code |
75015 |
| Country |
France |
| |
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| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
|
| Samples (3) |
| GSM850202 |
Endogenous small RNAs from human senescent fibroblast WI-38 |
| GSM850203 |
immunoprecipitating small RNA with an antibody againt AGO proteins from human senescent fibroblast WI-38 |
| GSM850204 |
immunoprecipitating small RNA with an antibody againt H3K9me2 proteins from human senescent fibroblast WI-38 |
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| Relations |
| SRA |
SRP009864 |
| BioProject |
PRJNA151319 |
