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| Status |
Public on Apr 01, 2012 |
| Title |
Sindbis virus induces the production of a novel class of endogenous siRNAs in Aedes aegypti mosquitoes |
| Organism |
Aedes aegypti |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
We examined the biogenesis of mRNA-derived endogenous short-interfering RNAs (endo-siRNAs) in the disease vector mosquito, Aedes aegypti. Under standard conditions, mRNA-derived endo-siRNAs were produced from the bidirectional transcription of tail-tail overlapping gene pairs. Upon infection with the alphavirus, Sindbis virus (SINV), another class of mRNA-derived endo-siRNAs was observed. Genes producing SINV-induced endo-siRNAs were not enriched for overlapping partners or nearby genes, but were enriched for transcripts with long 3'UTRs. Endo-siRNAs from this class derived uniformly from the entire length of the target transcript, and were found to regulate the transcript levels of the genes from which they were derived. Strand-specific qPCR experiments demonstrated that antisense strands of targeted mRNA genes were produced to exonic, but not intronic regions. Finally, small RNAs mapped to both sense and antisense strands of exon-exon junctions, suggesting double-stranded RNA precursors to SINV-induced endo-siRNAs may be synthesized from mature mRNA templates. These results suggest additional complexity in small RNA pathways and gene regulation in the presence of an infecting virus in disease vector mosquitoes.
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| Overall design |
Examination of endo-siRNA production in Aedes aegypti mosquitoes
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| Contributor(s) |
Adelman ZN, Anderson MA, Liu M, Zhang L, Myles KM |
| Citation(s) |
22458920 |
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| Submission date |
Jan 17, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Zach N. Adelman |
| E-mail(s) |
[email protected]
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| Phone |
540-231-6614
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| Organization name |
Virginia Tech
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| Department |
Entomology
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| Street address |
305 Fralin Life Science Institute
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| City |
Blacksburg |
| State/province |
VA |
| ZIP/Postal code |
24061 |
| Country |
USA |
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| Platforms (2) |
| GPL11087 |
Illumina Genome Analyzer (Aedes aegypti) |
| GPL14594 |
Illumina Genome Analyzer II (Aedes aegypti) |
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| Samples (3) |
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| Relations |
| SRA |
SRP010394 |
| BioProject |
PRJNA150789 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE35161_RAW.tar |
80.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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