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| Status |
Public on May 03, 2006 |
| Title |
Comparison of neural stem cell lines overexpressing Nurr1 to clonal controls |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The orphan nuclear receptor Nurr1 has been shown to be critical for the development of ventral midbrain dopaminergic neurons. Consequently, the development of ES cells overexpressing Nurr1 has raised hope for the development of cell replacement therapies for Parkinson's Disease to replace degenerated dopaminergic neurons. However, the molecular consequences of Nurr1 on gene expression in these cells remain unknown. To address this, stable, clonal, c17.2 neural stem cell lines were established that overexpressed the orphan nuclear receptor Nurr1 (clone 42 & clone 48) or parental control cell line (puroB & puroD, respectively).
Keywords: genetic modification
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| Overall design |
Stable neural stem cell lines were grown in proliferating conditions and matched for further microarray analysis based on their similar proliferation rates:
clone 42(c42) vs. puroB(pB)
clone 42(c48) vs. puroD(pD)
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| |
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| Contributor(s) |
Sousa KM, Mira H, Hall AC, Sjostrand LJ, Kusakabe M, Arenas E |
| Citation(s) |
17038671 |
| |
| Submission date |
Nov 06, 2005 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Kyle Matthew Sousa |
| Phone |
+46 8 5248 7866
|
| Fax |
+46 8 34 1960
|
| URL |
http://www.cajal.mbb.ki.se
|
| Organization name |
Molecular Neurobiology
|
| Department |
Medical Biochemistry and Biophysics
|
| Lab |
Karolinska Institute
|
| Street address |
Scheeles väg 1 A1 Plan 2
|
| City |
Stockholm |
| ZIP/Postal code |
17177 |
| Country |
Sweden |
| |
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| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA93649 |
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