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Series GSE35725 Query DataSets for GSE35725
Status Public on Sep 01, 2012
Title Transcriptional Signatures as a Disease-Specific and Predictive Inflammatory Biomarker for Type 1 Diabetes [T1D_114]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The complex milieu of inflammatory mediators associated with many diseases is often too dilute to directly measure in the periphery, necessitating development of more sensitive measurements suitable for mechanistic studies, earlier diagnosis, guiding selection of therapy, and monitoring interventions. Previously, we determined that plasma of recent-onset (RO) Type 1 diabetes (T1D) patients induce a proinflammatory transcriptional signature in fresh peripheral blood mononuclear cells (PBMC) relative to that of unrelated healthy controls (HC). Here, using an optimized cryopreserved PBMC-based protocol, we analyzed larger RO T1D and HC cohorts. In addition, we examined T1D progression by looking at longitudinal, pre-onset and longstanding T1D samples.
 
Overall design UPN727 cells were stimulated with autologous plasma (n=7), unrelated healthy control plasma (n=44), recent onset T1D plasma (n=46), longstanding T1D plasma (n=11), or longitudinal series plasma (n=6 time points) from pre/post onset. Gene expression analysis was performed in order to evaluate the transcriptional signature associated with T1D.
 
Contributor(s) Levy H, Wang X, Kaldunski M, Jia S, Kramer J, Pavletich SJ, Reske M, Quasney MW, Dahmer MK, Gorski J, Hessner MJ
Citation(s) 22972474, 24760139, 28143555
Submission date Feb 10, 2012
Last update date Sep 11, 2019
Contact name Martin Hessner
E-mail(s) [email protected]
Organization name Medical College of Wisconsin
Department Pediatrics
Lab Max McGee National Research Center for Juvenile Diabetes
Street address 8701 Watertown Plank Road
City Milwaukee
State/province WI
ZIP/Postal code 53226
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (114)
GSM874033 UPN727 cells, autologous plasma, biological replicate 1 [T1D]
GSM874034 UPN727 cells, unrelated healthy control 2074 plasma
GSM874035 UPN727 cells, unrelated healthy control 2302 plasma
This SubSeries is part of SuperSeries:
GSE35713 Transcriptional Signatures as a Disease-Specific and Predictive Inflammatory Biomarker for Type 1 Diabetes
Relations
BioProject PRJNA155659

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35725_RAW.tar 478.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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