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| Status |
Public on Dec 01, 2013 |
| Title |
Mutant-CCAAT/enhancer-binding protein alpha target genes in myeloid progenitor cells by chromatin IP |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
CCAAT/enhancer-binding protein alpha (C/EBPα) is a lineage-specific transcription factor that directs development of granulocytes. To obtain insights into the genome-wide transcriptome of mutant C/EBPα, we have performed chromatin immunoprecipitation on DNA promoter microarrays (ChIP-chip) in the murine 32D myeloid progenitor cell line. These cells expressed an estradiol-inducible form of mutant C/EBPα.
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| Overall design |
In the murine 32D myeloid progenitor cell line, expressing an estradiol-inducible form of C-terminal mutant of C/EBPα. (n=2, replicates). We have performed chromatin immunoprecipitation on DNA promoter microarrays (ChIP-chip) after 4 hours of estradiol incubation.
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| Contributor(s) |
Taskesen E, Beekman R, de Ridder J, Wouters BJ, Peeters JK, Touw I, Reinders MJ, Delwel R |
| Citation(s) |
23975790 |
| |
| Submission date |
Mar 05, 2012 |
| Last update date |
Oct 14, 2014 |
| Contact name |
Erdogan Taskesen |
| Organization name |
Erasmus University Medical Center (EMC)
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| Street address |
dr. molewaterplein 50
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| City |
Rotterdam |
| ZIP/Postal code |
3015GE |
| Country |
Netherlands |
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| Platforms (1) |
| GPL5811 |
[Mm_PromPR] Affymetrix Mouse Promoter 1.0R Array |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA153097 |
