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| Status |
Public on Jul 01, 2013 |
| Title |
Transcriptomes of transgenic rice expressing a constitutively active phytochrome OsPHYB-Y283H (OsYHB) allele |
| Platform organism |
Oryza sativa |
| Sample organism |
Oryza sativa Japonica Group |
| Experiment type |
Expression profiling by array
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| Summary |
The present study quantifies the transcriptomes of wild-type and transgenic Ubi::OsYHB rice seedlings (in the genetic background of Oryza sativa ssp. japonica CV Nipponbare) grown in the dark or under continous red light (Rc, at 50 µmol m-2 s-1) conditions.
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| Overall design |
WT (Nipponbare cultivar; Nip) and Ubi::OsYHB/Nip transgenic seedlings were grown at 28°C for 5 days in darkness or under continuous red light at 50 µmol m-2 s-1 (Rc50). Seedlings were harvested in subjective morning, immediately frozen in liquid nitrogen and strored at -80°C until RNA extraction.
The expression of OsYHB is driven by the maize Ubiquitin promoter.
Two biological replicates for each treatment. Two independent, genetically single-insertion, homozygous Ubi::OsYHB/Nip lines (#1 and # 9, with determined 41- and 23-fold overexpression levels of OsYHB in comparison to the wild-type control, respectively) were used.
GeneChip 3' IVT Express Kit (Affymetrix) was used to synthesize and label aRNA.
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| Contributor(s) |
Hu W, Lagarias JC |
| Citation(s) |
24961368, 32346668 |
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| Submission date |
Mar 06, 2012 |
| Last update date |
May 07, 2020 |
| Contact name |
Wei Hu |
| Organization name |
Univ of California at Davis
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| Department |
Department of Molecular and Cellular Biology
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| Lab |
J Clark Lagarias
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| Street address |
One Shields Ave.
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| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
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| Platforms (1) |
| GPL2025 |
[Rice] Affymetrix Rice Genome Array |
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| Samples (8)
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| GSM888883 |
WT (Nipponbare), Rc50, biol rep 2 |
| GSM888884 |
Ubi::OsYHB/Nip line #1, Dark, biol rep 1 |
| GSM888885 |
Ubi::OsYHB/Nip line #9, Dark, biol rep 2 |
| GSM888886 |
Ubi::OsYHB/Nip line #1, Rc50, biol rep 1 |
| GSM888887 |
Ubi::OsYHB/Nip line #9, Rc50, biol rep 2 |
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| Relations |
| BioProject |
PRJNA153365 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE36320_RAW.tar |
34.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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