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| Status |
Public on Sep 01, 2012 |
| Title |
The effect of blood on Fusobacterium nucleatum |
| Platform organism |
Fusobacterium nucleatum |
| Sample organism |
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 |
| Experiment type |
Expression profiling by array
|
| Summary |
Fusobacterium nucleatum is a Gram-negative oral bacterial species associated with periodontal disease progression. As periodontal disease progresses, it is known F. nucleatum coaggregated with blood is frequently detected in the gingival crevice. However, it is largely unknown whether these interactions between F. nucleatum and blood induce a particular genetic response. We tested the cultures of F. nucleatum ATCC 25586 with or without blood in a semi-defined growth medium by microarray analysis and found 14 genes that were affected after only about 4hr. of adhered blood. Then, we selected 7 genes that changed significantly and tested these genes via real-time RT-PCR to confirm the validity of the microarray results. As a result, one amino sugar-binding protein on the membrane of F. nucleatum was especially expressed high via both microarray and real-time RT-PCR. Based upon these data, it appears that the protein on the F. nucleatum membrane binds and transfers the amino sugar only under blood conditions.
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| Overall design |
This study aims to determine the effect of blood on the gene expression profiling of F. nucreatum. The study contains 2 separate experiments that both measure the cultures without or with blood. The samples with blood contain 9% and 33% blood.
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| Contributor(s) |
Yoneda S |
| Citation(s) |
24896093 |
| |
| Submission date |
Mar 10, 2012 |
| Last update date |
Jun 04, 2015 |
| Contact name |
Justin Merritt |
| E-mail(s) |
[email protected]
|
| Phone |
405 271-2324
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| Organization name |
University of Oklahoma Health Sciences Center
|
| Department |
Oral Biology
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| Street address |
975 NE 10th St BRC364
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| City |
Oklahoma City |
| State/province |
OK |
| ZIP/Postal code |
73104 |
| Country |
USA |
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| Platforms (1) |
| GPL9447 |
Affymetrix GeneChip Dentala520298F |
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| Samples (3) |
| GSM892837 |
Fusobacterium nucleatum in 0% blood |
| GSM892838 |
Fusobacterium nucleatum in 9% blood |
| GSM892839 |
Fusobacterium nucleatum in 33% blood |
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| Relations |
| BioProject |
PRJNA153209 |
