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Series GSE36884 Query DataSets for GSE36884
Status Public on Oct 02, 2012
Title Expression profile of wild-type Thermus thermophilus HB8 strain cultured in TT medium for 6 h, reproduced in 2011
Organism Thermus thermophilus HB8
Experiment type Expression profiling by array
Summary We observed the expression profile of the total mRNA of the wild-type strain grown in a rich medium at 70 deg-C for 360 min.
 
Overall design Four wild-type T. thermophilus HB8 strains were each pre-cultured at 70°C for 16 h in 4 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells were inoculated into 1 liter of the same medium to a final OD600nm of 0.0005 and then cultivated at 70°C. Cells were collected after 360 min (OD600nm =~ 1.4), and then, the expression of each mRNA was analyzed.
 
Contributor(s) Agari Y, Sakamoto K, Kuramitsu S, Shinkai A
Citation(s)
  • Agari Y, Sakamoto K, Kuramitsu S, Shinkai A. Transcriptional repression mediated by a TetR family protein, PfmR, from Thermus thermophilus HB8. J Bacteriol 2012 Sep;194(17):4630-41. PMID: 22753056
Submission date Mar 28, 2012
Last update date Dec 19, 2012
Contact name Akeo Shinkai
E-mail(s) ashinkai@spring8.or.jp, y_agari@spring8.or.jp
URL http://www.srg.harima.riken.jp/
Organization name RIKEN Harima Institute
Department SPring-8 Center
Street address 1-1-1, Kouto, Sayo
City Hyogo
ZIP/Postal code 679-5148
Country Japan
 
Platforms (1)
GPL9209 [TTHB8401a520105F] Riken Thermus thermophilus HB8 4K mRNA custom array, library Rev.2
Samples (4)
GSM904789 wild-type strain cultured for 360 min_1 (TT/2011)
GSM904790 wild-type strain cultured for 360 min_2 (TT/2011)
GSM904791 wild-type strain cultured for 360 min_3 (TT/2011)
This SubSeries is part of SuperSeries:
GSE36912 SuperSeries for the study of expression analysis of the PfmR (TTHB023) in T. thermophilus HB8
Relations
BioProject PRJNA157009

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36884_RAW.tar 7.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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