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Status |
Public on Sep 01, 2012 |
Title |
Gene expression profiling of MCF-7 cells treated with 6-shogaol |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
A total of 18 genes were up-regulated and 11 genes were down-regulated > 2-fold following 6-shogaol treatment
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Overall design |
Microarray experiments were performed in duplicate as follows: MCF-7 cells were treated with 10 uM of 6-shogaol or control vehicle (0.1% DMSO) for 48 h. Total RNA from cells was extracted using Qiagen RNA isolation kit (Qiagen, Valencia, CA, USA) according to the manufacturers’ protocol. RNA purity was examined by spectrophotometric determination at 260/280 nm. The microarray hybridizations were carried out using the Affymetrix Human Gene 1.0 ST arrays.
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Contributor(s) |
Leong C |
Citation missing |
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Submission date |
Apr 02, 2012 |
Last update date |
Jul 26, 2018 |
Contact name |
Chee-Onn Leong |
E-mail(s) |
[email protected]
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Organization name |
International Medical University
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Street address |
126 Jalan 19/155B
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City |
Bukit Jalil |
ZIP/Postal code |
57000 |
Country |
Malaysia |
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Platforms (1) |
GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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Samples (4)
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Relations |
BioProject |
PRJNA157451 |