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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 18, 2012 |
| Title |
Altered Subcellular Localization of Transcription Factor TEAD4 Regulates First Mammalian Cell Lineage Commitment |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In the preimplantation mouse embryo TEAD4 is critical to establishing the trophectoderm (TE)-specific transcriptional program and segregating TE from the inner cell mass (ICM). However, TEAD4 is expressed both in the TE and the ICM. Thus, differential function of TEAD4 rather than expression itself regulates specification of the first two cell lineages. We used ChIP-seq to define genome-wide TEAD4 target genes and asked how transcription of TEAD4 target genes is specifically maintained in the TE. Our analyses revealed an evolutionarily conserved mechanism, in which lack of nuclear localization of TEAD4 impairs the TE-specific transcriptional program in inner blastomeres, thereby allowing their maturation towards the ICM lineage. Restoration of TEAD4 nuclear localization maintains the TE-specific transcriptional program in the inner blastomeres and prevents segregation of the TE and ICM lineages and blastocyst formation. We propose that altered subcellular localization of TEAD4 in blastomeres dictates first mammalian cell fate specification.
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| Overall design |
ChIPseq profiles of TEAD4, IgG, Input in Mouse trophoblast stem cells using Illumina HiSeq 2000 and Illumina Genome Analyzer IIx
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| Contributor(s) |
Home P, Gunewardena S, Yoo B, Schulz V, Gallagher PG, Paul S |
| Citation(s) |
22529382 |
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| Submission date |
Apr 17, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Byunggil Yoo |
| E-mail(s) |
[email protected]
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| Organization name |
KUMC
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| Street address |
3901 Rainbow Blvd
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| City |
Kansas City |
| State/province |
KS |
| ZIP/Postal code |
66202 |
| Country |
USA |
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| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
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| Samples (3) |
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| Relations |
| SRA |
SRP012267 |
| BioProject |
PRJNA159725 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE37350_RAW.tar |
851.8 Mb |
(http)(custom) |
TAR (of BW) |
| GSE37350_peaks_tead4_e-5_4949.bb |
125.8 Kb |
(ftp)(http) |
BB |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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