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| Status |
Public on Jan 01, 2014 |
| Title |
MBD-Seq of the V/SVZ and the CP at E16 |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Cell differentiation and proliferation are mutually exclusive. Although differentiating neurons are recognized as post-mitotic non-dividing cells, some Rb- and Rb family (Rb, p107, and p130)-deficient differentiating neurons proliferate and form tumor. Here, we found that the acute inactivation of all Rb family in differentiating cortical excitatory neurons caused radial migration defect and S-phase progression but not cell division, whereas that in cortical progenitors caused the cell division of the differentiating neurons generated from Rb –/–; p107 –/–; p130 –/– (Rb-TKO) progenitors. Genome-wide DNA methylation analysis revealed that proximal promoters tended to become methylated during differentiation in vivo. DNA demethylation by DNA methyltransferase inhibitor allowed the acutely inactivated Rb-TKO differentiating neurons to undergo G2/M-phase progression. Our finding illustrate that cortical excitatory neurons epigenetically lose their proliferative potency after neurogenesis.
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| Overall design |
1 sample of the V/SVZ tissue and the CP tissue
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| |
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| Contributor(s) |
Ajioka I, Oshikawa M |
| Citation missing |
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| |
| Submission date |
Apr 26, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Itsuki Ajioka |
| Organization name |
Tokyo Medical and Dental University
|
| Department |
Center for Brain Integration Research
|
| Street address |
1-5-45 Yushima, Bunkyo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
113-8510 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE37625 |
V/SVZ (ventricular/subventricular zone) tissue and CP (cortical plate) tissue at E16 |
|
| Relations |
| BioProject |
PRJNA162421 |
| SRA |
SRP012554 |
