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| Status |
Public on May 01, 2013 |
| Title |
Fis occupancy along the Salmonella enterica serovar Typhimurium SL1344 fis::3XFlag strain grown in different aeration regimes. |
| Platform organism |
Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 |
| Sample organism |
Salmonella enterica subsp. enterica serovar Typhimurium |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
The regulatory role of the Fis protein in fis and in the transcription of several gene regions during mid-exponential and late-stationary phase, and during different growth aeration regimes, has been investigated. Studies were done during those two growth phases and in aerated and non-aerated (microaerobic) conditions, to measure Fis enrichment and binding peaks in strategic gene regions by genome-wide microarray analysis ChIP-chip. This research investigation points to central roles for SPI-1, SPI-2, DNA gyrase and topoisomerase I, the elements of the stringent response, and the regulatory function of Fis-binding patterns, in setting and re-setting the activity of the fis gene and other involved promoters as a function of the growth conditions and aeration regimes experienced by Salmonella.
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| Overall design |
One sample with four different treatments. Three biological replicates per sample. The Aerated 2 hour sample was set as the control condition for all samples.
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| Contributor(s) |
Guadarrama S, Hokamp K, Dillon S, Dorman C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 12, 2012 |
| Last update date |
May 02, 2013 |
| Contact name |
Karsten Hokamp |
| E-mail(s) |
[email protected]
|
| Phone |
+35318962719
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| Organization name |
Trinity College Dublin
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| Department |
Smurfit Institute of Genetics
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| Street address |
College Green
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| City |
Dublin |
| ZIP/Postal code |
2 |
| Country |
Ireland |
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| Platforms (1) |
| GPL10008 |
Oxford Gene Technology Chip2 Salmonella typhimurium microarray SL1344 strain product code: 010021 |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA168358 |
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