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| Status |
Public on Aug 15, 2013 |
| Title |
Liver gene expression profile _ wild type_ CYP7A1 transgenic |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Bile acids are not only physiological detergents facilitating nutrient absorption, but also signaling molecules regulating metabolic homeostasis. We reported recently that transgenic expression of CYP7A1 in mice stimulated bile acid synthesis and prevented Western diet-induced obesity, insulin resistance and hepatic steatosis. The aim of this experiment is to determine the impact of induction of hepatic bile acid synthesis on liver metabolism by determining hepatic gene expression profile in CYP7A1 transgenic mice. CYP7A1 transgenic mice and wild type control mice were fed either standard chow diet or high fat high cholesterol Western diet for 4 month. Hepatic gene expressions were measured by microarray analysis. Our results indicate that hepatic bile acid synthesis is closely linked to cholesterogenesis and lipogenesis, and maintaining bile acid homeostasis is improtant in hepatic metabolic homeostasis.
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| Overall design |
Male aged matched (~ 12-14 weeks) CYP7A1 transgenic mice and their wild type control littermates were fed a standard chow diet or a high fat (42%) high cholesterol (0.2%) diet (Harlan Teklad #88137) for 4 month Four groups (4 mice/group) are included in the experiments:
Group 1: WT _ Chow
Group 2: CYP7A1-tg + chow
Group 3: WT + Western diet
Group 4: CYP7A1-tg _ Western diet
Total liver mRNA was isolated with a RNeasy kit (Qiagen) and used for microarray analysis.
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| Contributor(s) |
Gopalan B, Boehme S, Cheng J, Gopalan B, Na J, Chiang J |
| Citation(s) |
23536474 |
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| Submission date |
Jun 22, 2012 |
| Last update date |
Jun 14, 2018 |
| Contact name |
John Chiang |
| E-mail(s) |
[email protected]
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| Organization name |
Northeast Ohio Medical University
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| Department |
Biochemistry and Molecular Pathology
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| Street address |
4209 State Route 44
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| City |
Rootstown |
| State/province |
OH |
| ZIP/Postal code |
44272 |
| Country |
USA |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA169150 |
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