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| Status |
Public on Dec 28, 2006 |
| Title |
EC_interferon |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
IFNs are highly pleiotropic cytokines also endowed with marked anti-angiogenic activity. In this study, the mRNA expression profiles of endothelial cells (EC) exposed in vitro to IFN-alpha, IFN-beta, or
IFN-gamma were determined. We found that in HUVEC as well as in other EC types 175 genes were upregulated (>2-fold increase) by IFNs, including genes involved in the host response to RNA viruses, inflammation, and apoptosis. Interestingly, 41 genes showed a >5-fold higher induction by IFN-alpha in EC compared to human fibroblasts; among them, the gene encoding the angiostatic chemokine CXCL11 was selectively induced by IFN-alpha in EC along with other genes associated
with angiogenesis regulation, including CXCL10, TRAIL, and guanylate binding protein 1 (GBP-1). These transcriptional changes were confirmed and extended by quantitative PCR analysis and ELISA; whereas IFN-alpha and IFN-beta exerted virtually identical effects on transcriptome modulation, a differential gene regulation by type I and type II IFN emerged, especially as far as quantitative aspects were concerned. In vivo, IFN-alpha-producing tumors over-expressed murine CXCL10-11,
GBP-1 and TRAIL, with evidence of CXCL11 production by tumor-associated EC. Overall, these findings improve our understanding of the anti-angiogenic effects of IFNs by showing that these cytokines trigger an anti-angiogenic transcriptional program in EC. Moreover, we suggest that quantitative differences in the magnitude of the transcriptional activation of IFNresponsive genes could form the basis for cell-specific transcriptional signatures.
Keywords: class comparison
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| Overall design |
comparison of interferon effects on endothelial cells (HUVEC, HMVEC) and fibrobloasts
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| Contributor(s) |
Indraccolo S, Minuzzo S, Pfeffer U, Gola E, Ferrari N |
| Citation(s) |
17202376, 19553003 |
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| Submission date |
Dec 28, 2005 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Ulrich Pfeffer |
| E-mail(s) |
[email protected]
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| Phone |
+390105558462
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| Organization name |
IRCCS AOU San Martino - IST
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| Lab |
Functional Genomics
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| Street address |
L. Benzi 10
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| City |
Genova |
| ZIP/Postal code |
16132 |
| Country |
Italy |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (23)
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| Relations |
| BioProject |
PRJNA94173 |
| Supplementary data files not provided |
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