|
| Status |
Public on Sep 12, 2012 |
| Title |
IP of 5-hydroxymethylcytosine (5-hmC) enriched DNA fragments from control and PB treated mouse livers (28 day) |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by genome tiling array
|
| Summary |
5-hmC is a novel epigenetic mark derived from oxidation of methylcytosine. Phenobarbital (PB) is a well studied non-genotoxic carcinogen with roles in epigenetic perturbation. We profile 5hmC in both control mouse livers as well as in the livers of 28 day PB treated mice. 5hmC is largely found to reside in the bodies of active genes and promoter levels are perturbed upon PB induced gene activation events.
5-hmC is enriched in the bodies of active genes as well as a subset of TSS regions associated with transcriptional silencing. Upon PB exposire a handfull of genes are induced which reveal increases in promoter 5hmC levels
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| |
|
| Overall design |
comparison of 5hmC profiles in 5 control and 5 PB exposed mouse livers
|
| |
|
| Contributor(s) |
Thomson JP, Hackett J |
| Citation(s) |
23598998, 23034186, 25985418 |
| |
| Submission date |
Aug 31, 2012 |
| Last update date |
Jun 10, 2019 |
| Contact name |
John Paterson Thomson |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Edinburgh
|
| Department |
MRC Human Genetics Unit
|
| Lab |
Meehan
|
| Street address |
Crewe Road
|
| City |
Edinburgh |
| ZIP/Postal code |
EH4 2XU |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL14890 |
NimbleGen Mouse 2.1M deluxe promoter array [100205_MM9_Promoter_MeDIP_HX1] |
|
| Samples (10)
|
| GSM995945 |
control mouse 1 liver 5hmC profile (28 d) |
| GSM995946 |
control mouse 2 liver 5hmC profile (28 d) |
| GSM995947 |
control mouse 3 liver 5hmC profile (28 d) |
| GSM995948 |
control mouse 4 liver 5hmC profile (28 d) |
| GSM995949 |
control mouse 5 liver 5hmC profile (28 d) |
| GSM995950 |
pb treated mouse 1 liver 5hmC profile (28 d) |
| GSM995951 |
pb treated mouse 2 liver 5hmC profile (28 d) |
| GSM995952 |
pb treated mouse 3 liver 5hmC profile (28 d) |
| GSM995953 |
pb treated mouse 4 liver 5hmC profile (28 d) |
| GSM995954 |
pb treated mouse 5 liver 5hmC profile (28 d) |
|
| This SubSeries is part of SuperSeries: |
| GSE40540 |
IP of 5-hydroxymethylcytosine (5-hmC) and 5-methylcytosine (5-mC) enriched DNA fragments from control and PB treated mouse livers |
|
| Relations |
| BioProject |
PRJNA174863 |
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