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| Status |
Public on Dec 01, 2012 |
| Title |
High-resolution transcriptome and genome-wide dynamics of RNA polymerase and NusA in Mycobacterium tuberculosis (ChIP-seq) |
| Organism |
Mycobacterium tuberculosis |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To construct a regulatory map of the genome of the human pathogen, Mycobacterium tuberculosis, we applied two complementary high-resolution approaches, strand-specific RNA-seq and ChIP-seq, to survey the global transcriptome and monitor genome-wide dynamics of RNA polymerase (RNAP) and NusA. ChIP-seq revealed that RNAP and the antiterminator, NusA, occurred ubiquitously with the NusA profile mirroring RNAP distribution in both the exponential and stationary phases of growth. Generally, promoter-proximal peaks for RNAP and NusA were observed, followed by a decrease in signal strength reflecting transcriptional polarity. Differential binding of RNAP and NusA in the two growth conditions correlated with transcriptional activity as reflected by RNA abundance. Indeed, a significant association between expression levels and the presence of NusA throughout the gene body was detected, confirming the peculiar transcription-promoting role of NusA. Integration of the datasets pinpointed transcriptional units, mapped promoters and uncovered new anti-sense and non-coding transcripts. Highly expressed transcriptional units were situated mainly on the leading strand, despite the relatively unbiased distribution of genes throughout the genome, thus helping the replicative and transcriptional complexes to align.
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| Overall design |
ChIP-Seq of RNAP and NusA in Mtb H37Rv at exponential and stationary phase cultures. Each experiment was performed in duplicate. Input DNA (No IP) was used as a control.
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| Contributor(s) |
Uplekar S, Rougemont J, Cole ST, Sala C |
| Citation(s) |
23222129 |
| |
| Submission date |
Sep 12, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Swapna Uplekar |
| Organization name |
EPFL
|
| Street address |
Station 19, EPFL-GHI-UPCOL
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL15138 |
Illumina Genome Analyzer IIx (Mycobacterium tuberculosis) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE40862 |
High-resolution transcriptome and genome-wide dynamics of RNA polymerase and NusA in Mycobacterium tuberculosis |
|
| Relations |
| BioProject |
PRJNA175134 |
| SRA |
SRP015744 |
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