|
| Status |
Public on Sep 25, 2012 |
| Title |
Comparison of transcriptome profile between wild-type and dao mutant plants |
| Platform organism |
Oryza sativa |
| Sample organism |
Oryza sativa Japonica Group |
| Experiment type |
Expression profiling by array
|
| Summary |
We identified and characterized that the rice Dioxygenase for Auxin Oxidation (dao) mutant displays pleiotropic phenotypes, including indehiscent anthers, sterile pollens, and increased free IAA levels in anthers.We conclude that DAO-mediated auxin catabolism is essential for auxin homeostasis and later stages of plant reproductive development, including anther dehiscence, and pollen viability.
We used microarrays to identify differentially expressed genes in dao.
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| Overall design |
For genome-wide expression analysis of dao, three replicates of WT and dao samples (RNA from mature anthers) were analyzed on Affymetrix Genechip® Rice Genome arrays by an Affymetrix service facility (CapitalBio Corporation) according to the manufacturer’s protocols. Genes showing a 2-fold change with a q-value ≤ 0.05 were considered to be differentially expressed.
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| |
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| Contributor(s) |
Zhao Z, Zhang Y, Wan J |
| Citation(s) |
24094741 |
| |
| Submission date |
Sep 24, 2012 |
| Last update date |
Dec 27, 2017 |
| Contact name |
Zhao Zhi Gang |
| E-mail(s) |
[email protected]
|
| Organization name |
Nanjing Agricultural University
|
| Department |
Institute of Crop Science
|
| Lab |
The National Key
|
| Street address |
Tongwei-load
|
| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
210095 |
| Country |
China |
| |
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| Platforms (1) |
| GPL2025 |
[Rice] Affymetrix Rice Genome Array |
|
| Samples (6)
|
| GSM1008508 |
wild type mature anther, biological rep1 |
| GSM1008509 |
wild type mature anther, biological rep2 |
| GSM1008510 |
wild type mature anther, biological rep3 |
|
| Relations |
| BioProject |
PRJNA175781 |
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