|
| Status |
Public on Oct 18, 2012 |
| Title |
Global analysis of Cdk9-dependence for VSV-induced genes in Drosophila cells |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
|
| Summary |
To determine the Cdk9 targets of VSV-induced genes in Drosophila cells at 4 hours post-infection
|
| |
|
| Overall design |
Drosophila cells were treated with Bgal or Cdk9 and infected with VSV (MOI 10) for 4 hours, in two independent biological replicates. Total RNA was isolated using Trizol and microarray experiments were performed at the University of Pennsylvania Microarray Facility (U. Penn MF) following directions according to the manufacturer’s protocol (Affymetrix). In brief, 100 ng RNA were amplified with the Ovation RNA Amplification System v2 (NuGen), the Encore Biotin Module (NuGen) was used for fragmentation and labeling, Protocol FS450 002 was used for hybridization, washing, and staining. Images were scanned using the GeneChip® Scanner 3000 and image analysis was performed using the Affymetrix® GeneChip® Command Console® Software (AGCC).
|
| |
|
| Contributor(s) |
Xu J, Tudor M, Cherry S |
| Citation(s) |
23084920 |
| |
| Submission date |
Oct 01, 2012 |
| Last update date |
May 04, 2018 |
| Contact name |
Sara Cherry |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Pennsylvania
|
| Street address |
304K Lynch Labs
|
| City |
Philadelphia |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
|
| Samples (4)
|
| GSM1012124 |
Bgal dsRNA and VSV infection at T4 hours_biological rep1 |
| GSM1012125 |
Bgal dsRNA and VSV infection at T4 hours_biological rep2 |
| GSM1012126 |
Cdk9 dsRNA and VSV infection at T4 hours_biological rep1 |
| GSM1012127 |
Cdk9 dsRNA and VSV infection at T4 hours_biological rep2 |
|
| Relations |
| BioProject |
PRJNA176305 |
Less...
More...