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| Status |
Public on Mar 15, 2006 |
| Title |
Comparison of Bradyrhizobium japonicum parent strain LO with irr mutant strain LODTM5 |
| Platform organism |
Bradyrhizobium diazoefficiens USDA 110 |
| Sample organism |
Bradyrhizobium japonicum |
| Experiment type |
Expression profiling by array
|
| Summary |
The purpose of the study is to identify Irr-responsive genes in the bacterium Bradyrhizobium japonicum. Parent strain LO was compared to irr mutant strain LODTM5 by whole genome microarray analysis. Both cell types were grown in iron-limited media.
Keywords: Comparison of B. japonicum wild type and mutant cells
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| Overall design |
RNA was isolated from three biological replicates for each cell type, and prepared for microarray analysis using Affymetrix chips. A complete description of these experiments are found in Yang et al., (2006) Mol. Microbiol. 60:427-437.
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| Contributor(s) |
Yang J, Sangwan I, Lindemann A, Hauser F, Hennecke H, Fischer H, O'Brian MR |
| Citation(s) |
16573691 |
| |
| Submission date |
Feb 02, 2006 |
| Last update date |
Jul 19, 2013 |
| Contact name |
Mark R. O'Brian |
| E-mail(s) |
[email protected]
|
| Phone |
716.829.3200
|
| Fax |
716.829.2725
|
| Organization name |
State University of New York at Buffalo
|
| Department |
Biochemistry
|
| Street address |
140 Farber Hall
|
| City |
Buffalo |
| State/province |
NY |
| ZIP/Postal code |
14214 |
| Country |
USA |
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| Platforms (1) |
| GPL3401 |
Affymetrix custom Bradyrhizobium japonicum strain USDA 110 19K array |
|
| Samples (6)
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| GSM94778 |
Bradyrhizobium japonicum strain LO, low iron, biological rep_1 |
| GSM94780 |
Bradyrhizobium japonicum strain LO, low iron, biological rep_2 |
| GSM94781 |
Bradyrhizobium japonicum strain LO, low iron, biological rep_3 |
| GSM94787 |
Bradyrhizobium japonicum irr mutant strain LODTM5, low iron, biological rep_1 |
| GSM94788 |
Bradyrhizobium japonicum irr mutant strain LODTM5, low iron, biological rep_2 |
| GSM94790 |
Bradyrhizobium japonicum irr mutant strain LODTM5, low iron, biological rep_3 |
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| This SubSeries is part of SuperSeries: |
| GSE4156 |
Iron- and Irr-regulated genes in Bradyrhizobium japonicum |
|
| Relations |
| BioProject |
PRJNA104611 |
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