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| Status |
Public on Jul 18, 2014 |
| Title |
Genome-wide translational changes induced by the prion [PSI+] |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
Expression profiling by high throughput sequencing
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| Summary |
Prions are infectious proteins that can adopt a structural conformation different from that of the normal protein. This change of conformation is then propagated among other molecules of the same protein. Prions are associated with neurodegenerative diseases in mammals, but are also found in fungi (in the yeast Saccharomyces cerevisiae and the filamentous fungus Podospora anserina), in which they control heritable traits. They are widespread in wild yeast strains, suggesting a biologically important role. [PSI+] is one of the most widely studied yeast prions. It corresponds to an aggregated conformation of the translational release factor, eRF3, which suppresses nonsense codons. [PSI+] modifies cellular fitness, inducing various phenotypes, depending on the genetic background. However, the genes displaying [PSI+]-controlled expression remain largely unknown. We used the recently described ribosome profiling approach to identify genes displaying changes in expression in the presence of [PSI+]. This made it possible to determine the positions of all active ribosomes within the genome, in both [PSI+] and [PSI-] isogenic strains. Comparisons of the translatomes and transcriptomes of the two strains revealed that the primary effect of [PSI+] was to repress genes involved in the stress response. Thus, we provide the first description of the global translational effect of [PSI+] and a new genetic explanation of the phenotypic differences between [PSI-] and [PSI+] strains under stress conditions.
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| Overall design |
Comparisons of the translatomes and transcriptomes of the two strains.
Comparisons of the translatomes of the two strains : a [PSI+] strain (two replicates) and a [PSI+] overexpressing SUP35-Cter strain.
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| Contributor(s) |
Baudin-Baillieu A, Legendre R, Kuchly C, Hatin I, Demais S, Mestdagh C, Gautheret D, Namy O |
| Citation(s) |
25043188 |
| |
| Submission date |
Oct 15, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Olivier Namy |
| E-mail(s) |
[email protected]
|
| Organization name |
CNRS, Université Paris-Sud
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| Department |
I2BC
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| Lab |
Genomic, structure and Translation
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| Street address |
Rue Gregor Mendel, bat 400
|
| City |
Orsay |
| ZIP/Postal code |
91400 |
| Country |
France |
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| Platforms (2) |
| GPL13272 |
Illumina Genome Analyzer IIx (Saccharomyces cerevisiae) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA177820 |
| SRA |
SRP016508 |
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