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| Status |
Public on Dec 06, 2012 |
| Title |
Expression data from wild type and ttk^twk mutant whole ovaries |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Epithelial tube morphogenesis requires precise orchestration of cell signaling, shape, migration, and adhesion. Follicle cells in the Drosophila ovary form epithelial tubes, the lumens of which act as molds for the eggshell respiratory filaments, or dorsal appendages (DAs). The Tramtrack69 (TTK69) transcription factor controls DA lumen volume and shape by promoting tube expansion; the tramtrack mutation twin peaks (ttk^twk) reduces TTK69 levels late in oogenesis, inhibiting DA tube expansion.
We used microarrays to identify differentially expressed genes in the ttk^twk mutant that are downstream of TTK69 and that play a functional role in DA tube expansion.
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| Overall design |
Ovaries were dissected from female flies at 36 hours of age, to maximize the proportion of developing egg chambers undergoing DA tube expansion. Flies were raised at a constant temperature of 25C in the presence of wet yeast, to maximize the rate of egg chamber production.
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| Contributor(s) |
Peters NC, Berg CA |
| Citation(s) |
23545328 |
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| Submission date |
Dec 05, 2012 |
| Last update date |
May 04, 2018 |
| Contact name |
Nathaniel Peters |
| E-mail(s) |
[email protected]
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| Phone |
2065431622
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| Fax |
2066857301
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| Organization name |
University of Washington
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| Department |
Genome Sciences
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| Lab |
Berg Lab
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| Street address |
3720 15th Ave NE, Box 355065
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| City |
Seattle |
| State/province |
Washington |
| ZIP/Postal code |
98195 |
| Country |
USA |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (6)
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| GSM1049480 |
36hr ttk^twk/ttk^twk ovary, biological rep1 |
| GSM1049481 |
36hr ttk^twk/ttk^twk ovary, biological rep2 |
| GSM1049482 |
36hr ttk^twk/ttk^twk ovary, biological rep3 |
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| Relations |
| BioProject |
PRJNA183069 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE42758_RAW.tar |
12.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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