|
| Status |
Public on Dec 26, 2012 |
| Title |
Protein Determinants of Meiotic DNA Break Hotspots (dataset 2) |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
Meiotic recombination, crucial for proper chromosome segregation and genome evolution, is initiated by programmed DNA double-strand breaks (DSBs) in budding and fission yeasts and likely all sexually reproducing species. In fission yeast, DSBs occur up to several hundred times more frequently at special sites, called hotspots, than in other regions of the genome. What distinguishes hotspots from cold regions is a major unsolved problem, although transcription factors determine some hotspots. We report here the discovery that three coiled-coil proteins -- Rec25, Rec27, and Mug20 -- bind essentially all hotspots with unprecedented, high specificity even without DSB formation. These small proteins are components of linear elements, are related to synaptonemal complex proteins, and are essential for nearly all DSBs at most hotspots. Our results indicate that these hotspot determinants activate or stabilize the DSB-forming protein Rec12 (Spo11 homolog) rather than promote its binding to hotspots. We propose here a new paradigm for hotspot determination and crossover control by linear element proteins.
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| Overall design |
16 ChIP-chip arrays of meiotic proteins at various times in meiosis and in several mutant backgrounds. Two protein pull-downs (Rec27 and Rec25) were repeated.
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| |
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| Contributor(s) |
Fowler KR, Smith GR |
| Citation(s) |
23395004 |
| |
| Submission date |
Dec 21, 2012 |
| Last update date |
Mar 27, 2013 |
| Contact name |
Kyle Fowler |
| Organization name |
University of California San Francisco
|
| Street address |
600 16th Street
|
| City |
San Francisco |
| State/province |
California |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16421 |
Agilent-025249 S. pombe Whole Genome ChIP-on-Chip Microarray 4x44K (Probe Name version) |
|
| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE43122 |
Protein Determinants of Meiotic DNA Break Hotspots |
|
| Relations |
| BioProject |
PRJNA184678 |
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