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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 21, 2013 |
| Title |
Transcriptome of mouse pluripotent embryonic stem cells (mESC) cultured in R2i, 2i, PD and SB conditions |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
In this study we have analyzed the global gene expression of naïve mouse embryonic stem cells in different culture conditions including R2i (PD0325901+SB431542), 2i (PD0325901+CHIR99021), and also PD0325901+LIF and SB431542+LIF to show the similarities and differences between the conditions in maintaining pluripotency.
Since the first generation of mouse embryonic stem (ES) cells, extrinsic regulation of pluripotency has been at the focus of attention. Here we show that suppression of transforming growth factor β (TGFβ) signaling could have impressive effects on self-renewal and pluripotency of mouse ES cells. We introduce a chemically defined medium with inhibitors of TGFβ and mitogen-activated protein kinase (MAPK) kinase, designated here as R2i (Royan 2 inhibitors), for highly efficient establishment of ES cell lines from various mouse strains. R2i also supports homogenous expression of Nanog and Dppa3 proteins in ES cells and shows minimal differentiation leakage. Our results uncover an appropriate condition for ES cell generation from single blastomeres of various cleavage-stage embryos. Further, the high accuracy of genome integrity after long-term cultivation in R2i illustrates an optimal condition for ES cell culture. Global transcriptomic analysis of R2i cells demonstrates that bone morphogenetic protein 4 (BMP4) signaling becomes overrepresented pursuant to TGFβ repression, which may confer further robustness to pluripotency through shielding cells from differentiation stimuli. These findings point to a new facet of ground state pluripotency by blocking differentiation pathways, without influencing the pluripotency regulatory circuitry of mouse ES cells.
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| Overall design |
Whole gene expression study of 4 different mouse embryonic stem cell lines maintained under 4 different chemically defined conditions: R2i, 2i, PD and SB
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| Contributor(s) |
Hassani SN, Totonchi M, Sharifi-Zarchi A, Mollamohammadi S, Pakzad M, Moradi S, Samadian A, Masoudi N, Mirshahvaladi S, Farrokhi A, Greber B, Araúzo-Bravo MJ, Sabour D, Sadeghi M, Salekdeh GH, Gourabi H, Schoeler H, Baharvand H |
| Citation(s) |
24036899 |
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| Submission date |
Jan 23, 2013 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Hossein Baharvand |
| E-mail(s) |
[email protected]
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| Phone |
+9821-22339936
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| Organization name |
Royan Institute for Stem Cell Biology and Technology
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| Street address |
Hafez St., Bani Hashem St.
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| City |
Tehran |
| ZIP/Postal code |
P.O.Box: 19395-4644 |
| Country |
Iran |
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| Platforms (2) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA187048 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE43682_ControlProbeProfile_Ref8-v2.txt.gz |
90.4 Kb |
(ftp)(http) |
TXT |
| GSE43682_ControlProbeProfile_WG6-v2.txt.gz |
44.2 Kb |
(ftp)(http) |
TXT |
| GSE43682_RAW.tar |
18.9 Mb |
(http)(custom) |
TAR |
| GSE43682_SampleProbeProfile_Ref8-v2.txt.gz |
2.7 Mb |
(ftp)(http) |
TXT |
| GSE43682_SampleProbeProfile_WG6-v2.txt.gz |
2.2 Mb |
(ftp)(http) |
TXT |
| GSE43682_Targets_Ref8-v2.txt.gz |
288 b |
(ftp)(http) |
TXT |
| GSE43682_Targets_WG6-v2.txt.gz |
318 b |
(ftp)(http) |
TXT |
| GSE43682_log2_norm_Ref8-v2.txt.gz |
1.5 Mb |
(ftp)(http) |
TXT |
| GSE43682_log2_norm_WG6-v2.txt.gz |
1.4 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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