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| Status |
Public on Sep 22, 2006 |
| Title |
Expression data from native ZAP-70+CD38+ vs. ZAP-70-CD38- CLL cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
B-cell chronic lymphocytic leukemia (B-CLL) is a heterogenous disease with a highly variable clinical course and analysis of ZAP-70 and CD38 expression on B-CLL cells allowed for identification of patients with good (ZAP-70-CD38-), intermediate (discordant expression of ZAP-70 and CD38) and poor (ZAP-70+CD38+) prognosis. In an attempt to identify a molecular basis that may underly this diverse clinical behaviour DNA microarray technology was employed to compare eight ZAP-70+CD38+ with eight ZAP-70-CD38- B-CLL cases.
We used microarrays to detail the global programme of gene expression distinguising B-CLL from patient with good (samples 1 to 8) and poor prognosis (sample 9 to 16) and identified distinct classes of up- and down-regulated genes.
Keywords: Disease progression
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| Overall design |
To compare the transcriptosomes of good prognosis CLL cases (ZAP-70-CD38-) to poor prognosis cases (ZAP-70+CD38+), we purified CD19+ cells from peripheral blood samples by immunomagnetic isolation using MidiMacs, resulting in >95% purity of leukemic cells as detected by FACS analysis of CD19+CD5+ cells. The leukemic cells were freshly purified from untreated patients and RNA was directly isolated from fresh cells without further ex vivo treatment of the cells. Eight immunomagnetically purified peripheral blood derived ZAP-70+CD38+ CLL cases were compared with eight ZAP-70-CD38- B-CLL cases.
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| Contributor(s) |
Huettmann A, Klein-Hitpass L, Thomale J, Deenen R, Carpinteiro A, Nueckel H, Edelmann J, Sellmann L, Duehrsen U, Duerig J |
| Citation(s) |
16932341 |
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| Submission date |
Mar 07, 2006 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Ludger Klein-Hitpass |
| E-mail(s) |
[email protected]
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| Phone |
+49 201 723 85552
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| Organization name |
Institut fuer Zellbiologie
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| Department |
Universitaetsklinikum
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| Lab |
BioChip Lab
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| Street address |
Virchowstr. 173
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| City |
Essen |
| ZIP/Postal code |
D-45122 |
| Country |
Germany |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (16)
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| GSM99339 |
B-CLL sample 653, good prognosis |
| GSM99340 |
B-CLL sample 623, good prognosis |
| GSM99341 |
B-CLL sample 813, good prognosis |
| GSM99342 |
B-CLL sample 708, good prognosis |
| GSM99343 |
B-CLL sample 705, good prognosis |
| GSM99344 |
B-CLL sample 621, good prognosis |
| GSM99345 |
B-CLL sample 783, good prognosis |
| GSM99346 |
B-CLL sample 658, good prognosis |
| GSM99347 |
B-CLL sample 777, poor prognosis |
| GSM99348 |
B-CLL sample 690, poor prognosis |
| GSM99349 |
B-CLL sample 838, poor prognosis |
| GSM99350 |
B-CLL sample 710, poor prognosis |
| GSM99351 |
B-CLL sample 844, poor prognosis |
| GSM99352 |
B-CLL sample 638, poor prognosis |
| GSM99353 |
B-CLL sample 1087, poor prognosis |
| GSM99354 |
B-CLL sample BB23, poor prognosis |
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| Relations |
| BioProject |
PRJNA94581 |
| Supplementary data files not provided |
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