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| Status |
Public on Oct 12, 2006 |
| Title |
Androgen receptor coregulators baseline comparisons |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
LNCaP cells are an established androgen receptor expressing prostate carcinoma cell line. Human foreskin fibroblasts also expressing the androgen receptor were obtained from phenotypic normal male individuals. Cells were cultured either at confluency leading to G0 cell cycle state or while they were proliferating. Cells were either untreated, or treated with dihydrotestosterone (DHT) or ethanol (ETOH) which also served as the solvent for the DHT. All experimental RNA samples derived from the untreated or treated cell lines were hybridized on cDNA arrays against a common reference. This reference was composed out of common reference CRG (50%) and out of fibroblast RNA (50%). This reference is also called "mixed reference" in the description of the 26 individual experiments.
A strain or line experiment design type assays differences between multiple strains, cultivars, serovars, isolates, lines from organisms of a single species.
Compound Based Treatment: dihydrotestosterone (DHT) or ethanol (ETOH = solvent)
Cell Line: genital fibroblast cell line or prostate carcinoma cell line (LNCaP)
Culture Synchrony: Go or proliferation
Keywords: strain_or_line_design
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| Overall design |
Computed
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| Contributor(s) |
Holterhus P |
| Citation(s) |
16932916 |
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| Submission date |
Mar 13, 2006 |
| Last update date |
Mar 17, 2012 |
| Contact name |
Paul-Martin Holterhus |
| E-mail(s) |
[email protected]
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| Phone |
+49 (0) 431-597 3959
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| Fax |
+49 (0) 431-597 3966
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| Organization name |
Universitätsklinikum Schleswig-Holstein
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| Department |
Pädiatrische Endokrinologie und Diabetologie
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| Lab |
Klinik für Allgemeine Pädiatrie
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| Street address |
Campus Kiel, Schwanenweg 20
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| City |
Kiel |
| ZIP/Postal code |
D-24105 |
| Country |
Germany |
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| Platforms (4)
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| Samples (26)
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| GSM100134 |
N-58, NORM, M, GSF, 1 x 100nM DHT, 24h |
| GSM100135 |
N-AKPZF-I, NORM, M, PRF |
| GSM100136 |
N-58, NORM, M, GSF, 1 x 1000nM DHT, 24h |
| GSM100137 |
LNCaP, 24h 100nM DHT/MR |
| GSM100138 |
N-33, NORM, M, GSF, 6 x ETOH, 14d |
| GSM100139 |
N-59, NORM, M, GSF |
| GSM100140 |
N-58, NORM, M, GSF, 6 x 100nM DHT, 14d |
| GSM100141 |
N-58, NORM, M, GSF |
| GSM100142 |
N-56, NORM, M, GSF |
| GSM100143 |
N-62, NORM, M, GSF |
| GSM100144 |
N-60, NORM, M, GSF |
| GSM100145 |
N-33, NORM, M, GSF |
| GSM100146 |
N-AKPZF-II, NORM, M, PRF |
| GSM100147 |
N-33, NORM, M, GSF, 6 x 100nM DHT, 14d |
| GSM100148 |
N-52, NORM, M, GSF |
| GSM100149 |
N-56, NORM, M, GSF, 6 x ETOH, 14d |
| GSM100150 |
N-56, NORM, M, GSF, 6 x 100nM DHT, 14d |
| GSM100151 |
N-58, NORM, M, GSF, 6 x ETOH, 14d |
| GSM100152 |
LNCaP, 24h ETOH/MR |
| GSM100153 |
LNCaP, 24h ETOH/MR-b |
| GSM100154 |
N-56, NORM, M, GSF, 1 x 100nM DHT, 24h |
| GSM100155 |
N-56, NORM, M, GSF, 1 x ETOH, 24h |
| GSM100156 |
N-56, NORM, M, GSF, 1 x 1000nM DHT, 24h |
| GSM100157 |
N-58, NORM, M, GSF, 1 x ETOH, 24h |
| GSM100158 |
N-40, NORM, M, GSF |
| GSM100159 |
LNCaP, 24h 1000nM DHT/MR |
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| This SubSeries is part of SuperSeries: |
| GSE4456 |
Androgen receptor coregulators |
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| Relations |
| BioProject |
PRJNA104673 |
| Supplementary data files not provided |
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