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| Status |
Public on Oct 12, 2006 |
| Title |
Androgen receptor-coregulators hormone responsiveness LNCaP / GSF |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The text description is: LNCaP are androgen receptor expressing prostate carcinoma cells. Genital skin fibroblasts also express the androgen receptor. Cells were either proliferating or they were G0-arrested. Treatment of cells was performed with either the androgen DHT (dihydrotestosterone), the androgen analogue R1881 (methyltrienolone), or the solvent ethanol. Some hybridizations were performed in the type 1 design. In these cases, the hormone treated sample was hybridized against the ethanol treated sample on the same microarray. Hormone mediated induction or repression of gene transcription can directly be deduced from R/G normalized ratios on arrays. For other experiments, the ethanol treated control and the hormone treated experiment were hybridized on separate microarrays against a common reference of RNA. This reference was composed out of common reference batch CRG (50%) and a fibroblast RNA (50%). This reference was called mixed reference. For all experiments, the same batch of mixed reference was used.
A compound treatment design type is where the response to administration of a compound or chemical (including biological compounds such as hormones) is assayed.
Compound Based Treatment: Ethanol, R1881 (methyltrienolone), or DHT (dihydrotestosterone)
Cell Line: LNCaP (prostate cancer) or foreskin fibroblasts
Culture Synchrony: proliferation / Go-arrest
Keywords: compound_treatment_design
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| Overall design |
Computed
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| Contributor(s) |
Holterhus P, DePrimo S |
| Citation(s) |
16932916 |
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| Submission date |
Mar 13, 2006 |
| Last update date |
Mar 17, 2012 |
| Contact name |
Paul-Martin Holterhus |
| E-mail(s) |
[email protected]
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| Phone |
+49 (0) 431-597 3959
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| Fax |
+49 (0) 431-597 3966
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| Organization name |
Universitätsklinikum Schleswig-Holstein
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| Department |
Pädiatrische Endokrinologie und Diabetologie
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| Lab |
Klinik für Allgemeine Pädiatrie
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| Street address |
Campus Kiel, Schwanenweg 20
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| City |
Kiel |
| ZIP/Postal code |
D-24105 |
| Country |
Germany |
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| Platforms (3) |
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| Samples (24)
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| GSM100134 |
N-58, NORM, M, GSF, 1 x 100nM DHT, 24h |
| GSM100136 |
N-58, NORM, M, GSF, 1 x 1000nM DHT, 24h |
| GSM100137 |
LNCaP, 24h 100nM DHT/MR |
| GSM100138 |
N-33, NORM, M, GSF, 6 x ETOH, 14d |
| GSM100140 |
N-58, NORM, M, GSF, 6 x 100nM DHT, 14d |
| GSM100147 |
N-33, NORM, M, GSF, 6 x 100nM DHT, 14d |
| GSM100149 |
N-56, NORM, M, GSF, 6 x ETOH, 14d |
| GSM100150 |
N-56, NORM, M, GSF, 6 x 100nM DHT, 14d |
| GSM100151 |
N-58, NORM, M, GSF, 6 x ETOH, 14d |
| GSM100152 |
LNCaP, 24h ETOH/MR |
| GSM100153 |
LNCaP, 24h ETOH/MR-b |
| GSM100154 |
N-56, NORM, M, GSF, 1 x 100nM DHT, 24h |
| GSM100155 |
N-56, NORM, M, GSF, 1 x ETOH, 24h |
| GSM100156 |
N-56, NORM, M, GSF, 1 x 1000nM DHT, 24h |
| GSM100157 |
N-58, NORM, M, GSF, 1 x ETOH, 24h |
| GSM100159 |
LNCaP, 24h 1000nM DHT/MR |
| GSM100160 |
LN24r1,10nM |
| GSM100161 |
LNCaP, 1000nM DHT, 24h |
| GSM100162 |
LN24DHT1000 |
| GSM100163 |
LN24r1,0.01 |
| GSM100164 |
LN24r1,0.1 |
| GSM100165 |
LN24r1,1 |
| GSM100166 |
LN24r1,0.001 |
| GSM100167 |
LN24DHT100 |
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| This SubSeries is part of SuperSeries: |
| GSE4456 |
Androgen receptor coregulators |
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| Relations |
| BioProject |
PRJNA104675 |
| Supplementary data files not provided |
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