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| Status |
Public on Jun 14, 2013 |
| Title |
Gene Expression in Bone-marrow derived Dendritic Cells stimulated with H. pylori lysate vs. E. coli lysate |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Gene Expression changes in BMDCs stimulated with H. pylori vs. E. coli. The hypothesis tested was that the gene expression profile in BMDCs stimulated with H. pylori lysate will be less inflammatory than BMDCs stimulated with E. coli.
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| Overall design |
BMDCs were generated from bone marrow of wild type female C57BL/6 mice using 100ng/mL Flt3L. DCs were harvested at day 8 and stimulated with either media alone, H. pylori antigen lysate or E. coli lysate. At 24 hours, the cells were harvested and RNA was isolated for microarray analysis.
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| Contributor(s) |
Blanchard T, Shiu J, Sun Y |
| Citation(s) |
23776703 |
| |
| Submission date |
Mar 07, 2013 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Jessica Shiu |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Maryland School of Medicine
|
| Department |
Pediatrics
|
| Lab |
Blanchard
|
| Street address |
655 W Baltimore St
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21201 |
| Country |
USA |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (8)
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| GSM1094372 |
BMDCs- Escherichia coli Ag lysate stimulated 2 |
| GSM1094373 |
BMDCs- Escherichia coli Ag lysate stimulated 3 |
| GSM1094374 |
BMDCs- Helicobacter pylori Ag lysate stimulated 1 |
| GSM1094375 |
BMDCs- Helicobacter pylori Ag lysate stimulated 2 |
| GSM1094376 |
BMDCs- Helicobacter pylori Ag lysate stimulated 3 |
|
| Relations |
| BioProject |
PRJNA192615 |
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