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| Status |
Public on Feb 12, 2014 |
| Title |
Transcriptional profiling of Pseudomonas aeruginosa PAO1161 strain and its parA and parB mutants |
| Organism |
Pseudomonas aeruginosa |
| Experiment type |
Expression profiling by array
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| Summary |
Representatives of two families of bacterial Par proteins, ParA and ParB, are encoded by the majority of bacterial chromosomes in the close vicinity of oriC. ParA(Soj) and ParB(Spo0J) proteins of Pseudomonas aeruginosa are both important for optimal growth, nucleoids segregation, cell division and different types of motility. Comparative transcriptome analysis of parAnull, parBnull mutants versus parental PAO1161 strain of P. aeruginosa demonstrated global changes in genes expression pattern in logarithmic phase of planktonic cultures grown on rich medium. The set of genes that were similarly regulated in both mutant strains as compared to the wild-type strain as well as two sets of genes uniquely affected in the particular mutant were defined suggesting that ParA and ParB may act in common and independently. In general, many genes involved in cell division, DNA and RNA processing and metabolic processes were down-regulated in mutant cells, in contrast genes which products play a role in adaptation, protection, motility, cell-to-cell signaling as well as signal transduction increased their expression in par mutant cells. Besides their role in chromosome segregation, ParA and ParB seem to have the potential to regulate genes transcription. The altered expression of a large number of genes encoding known or predicted transcriptional regulators and genes coding for products involved in c-di-GMP signalling, suggests that the part of observed global changes in genes expression pattern in parAnull and parBnull mutants might be the effect of indirect regulation mediated by regulatory genes under ParA and ParB control. The extended regulatory network provides the mechanism to modulate genes expression in response to the stage of the chromosome segregation process and cell cycle.
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| Overall design |
Pseudomonas aeruginosa PAO1161 (leu, r-, RifR), derivative of PAO1, as a control (reference) strain, Pseudomonas aeruginosa PAO1161 parA1-40::smh (parAnull) and Pseudomonas aeruginosa PAO1161 parB1-18::TcR (parBnull) disruption mutant strains were used in the experiments. Three independent biological replicates of total RNA were isolated for each strain from logarithmic (Log) phase of planktonic culture grown on rich medium (L broth) at 37oC. In total, nine samples of RNA were prepared.
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| Contributor(s) |
Bartosik AA, Jagura-Burdzy G, Fogtman A, Koblowska M |
| Citation(s) |
24498062 |
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| Submission date |
May 16, 2013 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Aneta Agnieszka Bartosik |
| E-mail(s) |
[email protected]
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| Phone |
+48 22 5921215
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| Organization name |
Polish Academy of Sciences
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| Department |
Institute of Biochemistry and Biophysics
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| Lab |
Depertment of Microbial Biochemistry
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| Street address |
Pawinskiego 5A
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| City |
Warsaw |
| ZIP/Postal code |
02-106 |
| Country |
Poland |
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| Platforms (1) |
| GPL84 |
[Pae_G1a] Affymetrix Pseudomonas aeruginosa Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA203313 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE47031_RAW.tar |
5.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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